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Systems Biology in Reproductive Medicine Volume 56, 2010 - Issue 1
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Research Communication

DNA Fragmentation Dynamics in Fresh Versus Frozen Thawed Plus Gradient-Isolated Human Spermatozoa

Jaime Gosálvez Department of Biology, Genetics Unit, Universidad Autónoma de Madrid, Madrid, SpainCorrespondence[email protected]
,
Joaquina de la Torre Department of Biology, Genetics Unit, Universidad Autónoma de Madrid, Madrid, Spain
,
Carmen López-Fernández Department of Biology, Genetics Unit, Universidad Autónoma de Madrid, Madrid, Spain
,
Laura Pérez-Gutiérrez Department of Biology, Genetics Unit, Universidad Autónoma de Madrid, Madrid, Spain
,
Leonor Ortega Tambre Clinic, Madrid, Spain
,
Pedro Caballero Tambre Clinic, Madrid, Spain
&
Rocio Nuñez Tambre Clinic, Madrid, Spain
 show all
Pages 27-36 | Received 17 Sep 2009, Accepted 18 Oct 2009, Published online: 19 Feb 2010

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Pascual Sánchez-Martín, Fernando Sánchez-Martín, Mercedes González-Martínez & Jaime Gosálvez. (2013) Increased pregnancy after reduced male abstinence. Systems Biology in Reproductive Medicine 59:5, pages 256-260.
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Articles from other publishers (27)

L B Belardin, M P Antoniassi, M Camargo, P Intasqui & R P Bertolla. (2022) Separating the chaff from the wheat: antibody-based removal of DNA-fragmented sperm. Human Reproduction.
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Arumugam Kumaresan, Mohua Das Gupta, Tirtha Kumar Datta & Jane M. Morrell. (2020) Sperm DNA Integrity and Male Fertility in Farm Animals: A Review. Frontiers in Veterinary Science 7.
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Ahmad Majzoub, Ashok Agarwal & Sandro C. Esteves. (2019) Clinical utility of sperm DNA damage in male infertility. Panminerva Medica 61:2.
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Aziza A. Saad, Tarek Hussein, Amany El-Sikaily, Mohamed A. Abdel-Mohsen, El-Hassan Mokhamer, Amany I. Youssef & Jihan Mohammed. (2019) Effect of Polycyclic Aromatic Hydrocarbons Exposure on Sperm DNA in Idiopathic Male Infertility. Journal of Health and Pollution 9:21.
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Kate Pollock, Jaime Gosálvez, Carmen López-Fernández & Stephen D. Johnston. (2018) Amphibian Sperm Chromatin Structure and Function and Its Relevance to Sperm Preservation. Journal of Herpetology 52:4, pages 486-491.
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Jaime Gosálvez, Ashok Agarwal & Sandro C. Esteves. 2018. A Clinician's Guide to Sperm DNA and Chromatin Damage. A Clinician's Guide to Sperm DNA and Chromatin Damage 571 587 .
Sezgin Gunes & Semra Sertyel. 2018. A Clinician's Guide to Sperm DNA and Chromatin Damage. A Clinician's Guide to Sperm DNA and Chromatin Damage 321 346 .
S. D. Johnston, C. López-Fernández, F. Arroyo, J. L. Fernández & J. Gosálvez. (2017) The assessment of sperm DNA fragmentation in the saltwater crocodile (Crocodylus porosus). Reproduction, Fertility and Development 29:3, pages 630.
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Robin W. Cotton & Matthew B. Fisher. (2015) Review: Properties of sperm and seminal fluid, informed by research on reproduction and contraception. Forensic Science International: Genetics 18, pages 66-77.
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Evangelini Evgeni, George Lymberopoulos, Maria Gazouli & Byron Asimakopoulos. (2015) Conventional semen parameters and DNA fragmentation in relation to fertility status in a Greek population. European Journal of Obstetrics & Gynecology and Reproductive Biology 188, pages 17-23.
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Jaime Gosálvez, Stephen Johnston, Carmen López-Fernández, Altea Gosálbez, Francisca Arroyo, Jose Luís Fernández & Juan GÁlvarez. (2014) Sperm fractions obtained following density gradient centrifugation in human ejaculates show differences in sperm DNA longevity. Asian Pacific Journal of Reproduction 3:2, pages 116-120.
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J. Ribas-Maynou, A. Fernández-Encinas, A. García-Peiró, E. Prada, C. Abad, M. J. Amengual, J. Navarro & J. Benet. (2014) Human semen cryopreservation: a sperm DNA fragmentation study with alkaline and neutral Comet assay. Andrology 2:1, pages 83-87.
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Jaime Gosálvez, Pedro Caballero, Carmen López-Fernández, Leonor Ortega, José Andrés Guijarro, José Luís Fernández, Stephen D Johnston & Rocío Nuñez-Calonge. (2013) Can DNA fragmentation of neat or swim-up spermatozoa be used to predict pregnancy following ICSI of fertile oocyte donors?. Asian Journal of Andrology 15:6, pages 812-818.
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Natalia Rougier, Heydy Uriondo, Sergio Papier, Miguel Angel Checa, Carlos Sueldo & Cristian Alvarez Sedó. (2013) Changes in DNA fragmentation during sperm preparation for intracytoplasmic sperm injection over time. Fertility and Sterility 100:1, pages 69-74.
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Jaime Gosálvez, Carlos García-Ochoa, Miguel Ruíz-Jorro, Manuel Martínez-Moya, Pascual Sánchez-Martín & Pedro Caballero. (2013) ¿A qué velocidad «muere» el ácido desoxirribonucleico del espermatozoide tras descongelar muestras seminales procedentes de donantes?. Revista Internacional de Andrología 11:3, pages 85-93.
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Carmen López-Fernández, Stephen D. Johnston, Altea Gosálbez, Jose Luís Fernández, Juan G. Álvarez & Jaime Gosálvez. (2013) Inter-center variation in the efficiency of sperm DNA damage reduction following density gradient centrifugation. Natural Science 05:07, pages 15-20.
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Jaime Gosálvez, Carmen López-Fernández & José Luís Fernández. 2013. Sperm Chromatin for the Researcher. Sperm Chromatin for the Researcher 257 281 .
Rocio Nuñez-Calonge, Pedro Caballero, Carmen López-Fernández, J. Andrés Guijarro, José Luís Fernández, Stephen Johnston & Jaime Gosálvez. (2012) An Improved Experimental Model for Understanding the Impact of Sperm DNA Fragmentation on Human Pregnancy Following ICSI. Reproductive Sciences 19:11, pages 1163-1168.
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Clara González-Marín, Jaime Gosálvez & Rosa Roy. (2012) Types, Causes, Detection and Repair of DNA Fragmentation in Animal and Human Sperm Cells. International Journal of Molecular Sciences 13:12, pages 14026-14052.
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S. D. Johnston, Y. P. Zee, C. Lopez-Fernandez & J. Gosalvez. (2012) The Effect of Chilled Storage and Cryopreservation on the Sperm DNA Fragmentation Dynamics of a Captive Population of Koalas. Journal of Andrology 33:5, pages 1007-1015.
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S. Karoui, C. Díaz, C. González-Marín, M. E. Amenabar, M. Serrano, E. Ugarte, J. Gosálvez, R. Roy, C. López-Fernández & M. J. Carabaño. (2012) Is sperm DNA fragmentation a good marker for field AI bull fertility?1. Journal of Animal Science 90:8, pages 2437-2449.
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Rebeca Santiso, María Tamayo, Jaime Gosálvez, Steve Johnston, Alfonso Mariño, Carlos Fernández, Carlos Losada & José Luis Fernández. (2012) DNA fragmentation dynamics allows the assessment of cryptic sperm damage in human: Evaluation of exposure to ionizing radiation, hyperthermia, acidic pH and nitric oxide. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 734:1-2, pages 41-49.
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Suseela Yelumalai, Junaid Kashir, Celine Jones, Hani Bagheri, Su Lin Oo, Laura McLaren & Kevin Coward. (2012) Clinician-induced (iatrogenic) damage incurred during human infertility treatment: Detrimental effects of sperm selection methods and cryopreservation upon the viability, DNA integrity, and function of human sperm. Asian Pacific Journal of Reproduction 1:1, pages 69-75.
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María Enciso, Miriam Iglesias, Isabel Galán, Jonás Sarasa, Antonio Gosálvez & Jaime Gosálvez. (2011) The ability of sperm selection techniques to remove single- or double-strand DNA damage. Asian Journal of Andrology 13:5, pages 764-768.
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J. Gosálvez, M.A. Ramirez, C. López-Fernández, F. Crespo, K.M. Evans, M.E. Kjelland & J.F. Moreno. (2011) Sex-sorted bovine spermatozoa and DNA damage: II. Dynamic features. Theriogenology 75:2, pages 206-211.
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Agustín García-Peiró, Juan Martínez-Heredia, María Oliver-Bonet, Carlos Abad, María José Amengual, Joaquima Navarro, Celine Jones, Kevin Coward, Jaime Gosálvez & Jordi Benet. (2011) Protamine 1 to protamine 2 ratio correlates with dynamic aspects of DNA fragmentation in human sperm. Fertility and Sterility 95:1, pages 105-109.
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Jaime Gosálvez, Carmen López-Fernández & José Luís Fernández. 2011. Sperm Chromatin. Sperm Chromatin 151 170 .

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