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Infection Ecology & Epidemiology Volume 6, 2016 - Issue 1

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Detection of Echinococcus multilocularis by MC-PCR: evaluation of diagnostic sensitivity and specificity without gold standard

Helene WahlströmDepartment of Disease Control and Epidemiology, National Veterinary Institute, Uppsala, SwedenCorrespondence[email protected]

, DVM, PhD, Dipl. ECVPH,

Arianna CominDepartment of Disease Control and Epidemiology, National Veterinary Institute, Uppsala, Sweden

, DVM, PhD,

Mats IsakssonDepartment of Microbiology, National Veterinary Institute, Uppsala, Sweden

Peter DeplazesInstitute of Parasitology, Vetsuisse and Medical Faculty, University of Zurich, Zurich, Switzerland

, Prof. Dr. med. vet., EVPC

Article: 30173 | Received 26 Oct 2015, Accepted 28 Jan 2016, Published online: 09 Mar 2016

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https://doi.org/10.3402/iee.v6.30173
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Mahboubeh Hadipour, Hossein Yousofi Darani, Hamid Talebzadeh, Mohammad Eslamian, Shima Aboutalebian, Majid Fasihi Harandi & Hossein Mirhendi. (2023) Sensitive detection of specific cell-free DNA in serum samples from sheep with cystic echinococcosis. PLOS Neglected Tropical Diseases 17:10, pages e0011715.
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Mughees Aizaz Alvi, Rana Muhammad Athar Ali, Sadiq Khan, Muhammad Saqib, Warda Qamar, Li Li, Bao-Quan Fu, Hong-Bin Yan & Wan-Zhong Jia. (2023) Past and Present of Diagnosis of Echinococcosis: A Review (1999-2021). Acta Tropica, pages 106925.
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Jonathon D. Kotwa, Tyler Greer, Claire M. Jardine, J. Scott Weese, Mats Isaksson, David L. Pearl, Olaf Berke, Nicola Mercer & Andrew S. Peregrine. (2020) Evaluation of the prevalence of Echinococcus multilocularis in dogs that visit off‐leash dog parks in southern Ontario, Canada . Zoonoses and Public Health 68:5, pages 533-537.
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Fabian Bagó, Franz Hoelzl, Felix Knauer, Anna Kübber-Heiss & Steve Smith. (2020) Rapid and Reliable Detection of Echinococcus multilocularis from Faeces Using Droplet Digital PCR. Acta Parasitologica 66:2, pages 553-559.
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Pervin Elvan TOKGÜN, Nuray ALTINTAŞ, Onur TOKGÜN & Nazmiye ALTINTAŞ. (2021) Echinococcus granulosus İnfeksiyonu Tanısında Moleküler Uygulamalar ve Yeni YaklaşımlarEchinococcus granulosus İnfeksiyonu Tanısında Moleküler Uygulamalar ve Yeni Yaklaşımlar. Van Sağlık Bilimleri Dergisi 14:1, pages 114-121.
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Piero Bonelli, Federica Loi, Maria Giovanna Cancedda, Angela Peruzzu, Elisabetta Antuofermo, Elisabetta Pintore, Toni Piseddu, Giovanni Garippa & Giovanna Masala. (2020) Bayesian Analysis of Three Methods for Diagnosis of Cystic Echinococcosis in Sheep. Pathogens 9:10, pages 796.
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Jonathon D. Kotwa, Mats Isaksson, Claire M. Jardine, G. Douglas Campbell, Olaf Berke, David L. Pearl, Nicola J. Mercer, Eva Osterman-Lind & Andrew S. Peregrine. (2019) Echinococcus multilocularis Infection, Southern Ontario, Canada . Emerging Infectious Diseases 25:2, pages 265-272.
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Maria A. Santa, Sonya Pastran, Claudia Klein, Kathreen Ruckstuhl & Alessandro Massolo. (2018) Evaluation of an automated magnetic bead-based DNA extraction and real-time PCR in fecal samples as a pre-screening test for detection of Echinococcus multilocularis and Echinococcus canadensis in coyotes. Parasitology Research 118:1, pages 119-125.
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P.S. Craig, D. Hegglin, M.W. Lightowlers, P.R. Torgerson & Q. Wang. 2017. Echinococcus and Echinococcosis, Part B. Echinococcus and Echinococcosis, Part B 55 158 .

Andrea L. Miller, Gert E. Olsson, Sofia Sollenberg, Moa Skarin, Helene Wahlström & Johan Höglund. (2016) Support for targeted sampling of red fox (Vulpes vulpes) feces in Sweden: a method to improve the probability of finding Echinococcus multilocularis. Parasites & Vectors 9:1.
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Miriam Maas, Annika van Roon, Cecile Dam-Deisz, Marieke Opsteegh, Alessandro Massolo, Gunita Deksne, Peter Teunis & Joke van der Giessen. (2016) Evaluation by latent class analysis of a magnetic capture based DNA extraction followed by real-time qPCR as a new diagnostic method for detection of Echinococcus multilocularis in definitive hosts. Veterinary Parasitology 230, pages 20-24.
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Detection of Echinococcus multilocularis by MC-PCR: evaluation of diagnostic sensitivity and specificity without gold standard

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Detection of Echinococcus multilocularis by MC-PCR: evaluation of diagnostic sensitivity and specificity without gold standard

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