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Of [hamsters] and men

A new perspective on host cell proteins

, &
Pages 1172-1174 | Received 13 Aug 2012, Accepted 13 Aug 2012, Published online: 01 Sep 2012

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (4)

Ann-Muriel Steff, Chanel Cadieux-Dion, Gaël de Lannoy, Maria Key Prato, Xavier Czeszak, Bruno André, Dominique C Ingels, Marc Louckx, Walthère Dewé, Marta Picciolato, Koen Maleux, Laurence Fissette & Ilse Dieussaert. (2020) Hamster neogenin, a host-cell protein contained in a respiratory syncytial virus candidate vaccine, induces antibody responses in rabbits but not in clinical trial participants. Human Vaccines & Immunotherapeutics 16:6, pages 1327-1337.
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Deepali Rathore, Anneliese Faustino, John Schiel, Eric Pang, Michael Boyne & Sarah Rogstad. (2018) The role of mass spectrometry in the characterization of biologic protein products. Expert Review of Proteomics 15:5, pages 431-449.
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James A Madsen, Victor Farutin, Theresa Carbeau, Steve Wudyka, Yan Yin, Stephen Smith, James Anderson & Ishan Capila. (2015) Toward the complete characterization of host cell proteins in biotherapeutics via affinity depletions, LC-MS/MS, and multivariate analysis. mAbs 7:6, pages 1128-1137.
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Catherine EM Hogwood, Daniel G Bracewell & C Mark Smales. (2013) Host cell protein dynamics in recombinant CHO cells. Bioengineered 4:5, pages 288-291.
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Articles from other publishers (32)

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Abdulrahman Tuameh, Stephen E. Harding & Nicholas J. Darton. (2022) Methods for addressing host cell protein impurities in biopharmaceutical product development. Biotechnology Journal 18:3.
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Haibin Luo, Qun Du, Chen Qian, Michelle Mlynarczyk, Timothy M. Pabst, Melissa Damschroder, Alan K. Hunter & William K. Wang. (2022) Formation of transient highly-charged mAb clusters strengthens interactions with host cell proteins and results in poor clearance of host cell proteins by protein A chromatography. Journal of Chromatography A 1679, pages 463385.
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Solomon Isu, Xianghong Qian, Andrew L. Zydney & S. Ranil Wickramasinghe. (2022) Process- and Product-Related Foulants in Virus Filtration. Bioengineering 9:4, pages 155.
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Song Nie, Tyler Greer, Reid O’Brien Johnson, Xiaojing Zheng, Albert Torri & Ning Li. (2021) Simple and Sensitive Method for Deep Profiling of Host Cell Proteins in Therapeutic Antibodies by Combining Ultra-Low Trypsin Concentration Digestion, Long Chromatographic Gradients, and BoxCar Mass Spectrometry Acquisition. Analytical Chemistry 93:10, pages 4383-4390.
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Patrick G. Schweickert, Ning Wang, Stephanie L. Sandefur, Michael E. Lloyd, Stephen F. Konieczny, Christopher. C. Frye & Zhuo Cheng. (2021) CRISPR/Cas12a‐mediated CHO genome engineering can be effectively integrated at multiple stages of the cell line generation process for bioproduction. Biotechnology Journal, pages 2000308.
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Wolfgang Esser-Skala, Marius Segl, Therese Wohlschlager, Veronika Reisinger, Johann Holzmann & Christian G. Huber. (2020) Exploring sample preparation and data evaluation strategies for enhanced identification of host cell proteins in drug products of therapeutic antibodies and Fc-fusion proteins. Analytical and Bioanalytical Chemistry 412:24, pages 6583-6593.
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Reid O’Brien Johnson, Tyler Greer, Milos Cejkov, Xiaojing Zheng & Ning Li. (2020) Combination of FAIMS, Protein A Depletion, and Native Digest Conditions Enables Deep Proteomic Profiling of Host Cell Proteins in Monoclonal Antibodies. Analytical Chemistry 92:15, pages 10478-10484.
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Vibha Jawa, Frances Terry, Jochem Gokemeijer, Shibani Mitra-Kaushik, Brian J. Roberts, Sophie Tourdot & Anne S. De Groot. (2020) T-Cell Dependent Immunogenicity of Protein Therapeutics Pre-clinical Assessment and Mitigation–Updated Consensus and Review 2020. Frontiers in Immunology 11.
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Patrick G. Schweickert & Zhuo Cheng. (2019) Application of Genetic Engineering in Biotherapeutics Development. Journal of Pharmaceutical Innovation 15:2, pages 232-254.
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Jong Youn Baik, Jing Guo & Kelvin H. Lee. 2019. Cell Culture Engineering. Cell Culture Engineering 295 311 .
Nobuo Fukuda, Yukako Senga & Shinya Honda. (2019) Anxa2 ‐ and Ctsd ‐knockout CHO cell lines to diminish the risk of contamination with host cell proteins . Biotechnology Progress 35:4.
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Madolyn L. MacDonald, Nathaniel K. Hamaker & Kelvin H. Lee. (2018) Bioinformatic analysis of Chinese hamster ovary host cell protein lipases. AIChE Journal 64:12, pages 4247-4254.
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Kristin N Valente, Nicholas E Levy, Kelvin H Lee & Abraham M Lenhoff. (2018) Applications of proteomic methods for CHO host cell protein characterization in biopharmaceutical manufacturing. Current Opinion in Biotechnology 53, pages 144-150.
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Cher Hui Goey, Sakhr Alhuthali & Cleo Kontoravdi. (2018) Host cell protein removal from biopharmaceutical preparations: Towards the implementation of quality by design. Biotechnology Advances 36:4, pages 1223-1237.
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James B. McGivneyIVIV, Andrew T. Csordas, Faye M. Walker, Elizabeth R. Bagley, Emily M. Gruber, Peter L. Mage, Jose Casas-Finet, Margaret A. Nakamoto, Michael Eisenstein, Christopher J. Larkin, Robert J. Strouse & H. Tom Soh. (2018) Strategy for Generating Sequence-Defined Aptamer Reagent Sets for Detecting Protein Contaminants in Biotherapeutics. Analytical Chemistry 90:5, pages 3262-3269.
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Amareth Lim, Brandon L. Doyle, Gerard M. Kelly, Angelia M. Reed-Bogan, Lawrence H. Breen, Parviz A. Shamlou & Peter K. Lambooy. (2018) Characterization of a cathepsin D protease from CHO cell-free medium and mitigation of its impact on the stability of a recombinant therapeutic protein. Biotechnology Progress 34:1, pages 120-129.
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Amit K. Dutta, Dmitriy Fedorenko, Jasmine Tan, Joseph A. Costanzo, David S. Kahn, Andrew L. Zydney & Oleg Shinkazh. (2017) Continuous countercurrent tangential chromatography for mixed mode post-capture operations in monoclonal antibody purification. Journal of Chromatography A 1511, pages 37-44.
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Deepti Ahluwalia, Harbhajan Dhillon, Thomas Slaney, Hangtian Song, Heather Boux, Sheetal Mehta, Lei Zhang, Anulfo Valdez & Girija Krishnamurthy. (2017) Identification of a host cell protein impurity in therapeutic protein, P1. Journal of Pharmaceutical and Biomedical Analysis 141, pages 32-38.
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Yifeng Li. (2017) Effective strategies for host cell protein clearance in downstream processing of monoclonal antibodies and Fc-fusion proteins. Protein Expression and Purification 134, pages 96-103.
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Josephine Chiu, Kristin N. Valente, Nicholas E. Levy, Lie Min, Abraham M. Lenhoff & Kelvin H. Lee. (2016) Knockout of a difficult‐to‐remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations. Biotechnology and Bioengineering 114:5, pages 1006-1015.
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P. Cheung, A. Emanuel, J. Heward, J. Maddalena, D. Toth, L. Saward & S. Kodihalli. (2016) Reduced immunogenic response to residual CHO cell protein in recombinant factor IX ( IB 1001) drug product in normal healthy rabbits . Haemophilia 22:3.
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Sampath R. Kumar. (2015) Industrial production of clotting factors: Challenges of expression, and choice of host cells. Biotechnology Journal 10:7, pages 995-1004.
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Catherine EM Hogwood, Daniel G Bracewell & C Mark Smales. (2014) Measurement and control of host cell proteins (HCPs) in CHO cell bioprocesses. Current Opinion in Biotechnology 30, pages 153-160.
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Chris Bailey‐Kellogg, Andres H. Gutiérrez, Leonard Moise, Frances Terry, William D. Martin & Anne S. De Groot. (2014) CHOPPI: A web tool for the analysis of immunogenicity risk from host cell proteins in CHO‐based protein production. Biotechnology and Bioengineering 111:11, pages 2170-2182.
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Nabila Aboulaich, Wai Keen Chung, Jenny Heidbrink Thompson, Christopher Larkin, David Robbins & Min Zhu. (2014) A novel approach to monitor clearance of host cell proteins associated with monoclonal antibodies. Biotechnology Progress 30:5, pages 1114-1124.
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Vibha Jawa, Leslie P. Cousens, Michel Awwad, Eric Wakshull, Harald Kropshofer & Anne S. De Groot. (2013) T-cell dependent immunogenicity of protein therapeutics: Preclinical assessment and mitigation. Clinical Immunology 149:3, pages 534-555.
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Daniel G BracewellC Mark Smales. (2013) The challenges of product- and process-related impurities to an evolving biopharmaceutical industry. Bioanalysis 5:2, pages 123-126.
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Haibin Luo, Qun Du, Chen Qian, Michelle Mlynarczyk, Timothy M. Pabst, Melissa Damschroder, Alan K. Hunter & William K. Wang. (2022) Formation of Transient Highly-Charged Mab Clusters Strengthens Interactions with Host Cell Proteins (Hcps) and Results in Poor Hcp Removal by Protein a Chromatography. SSRN Electronic Journal.
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