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High-affinity monoclonal antibodies to cell surface tumor antigen glypican-3 generated through a combination of peptide immunization and flow cytometry screening

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Pages 592-599 | Published online: 23 Jul 2012

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Crossref
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Crossref
Nicole H. Trier & Gunnar Houen. 2017. 43 96 .
Yi-Fan Zhang, Jessica Hong & Mitchell Ho. 2017. Immunotherapy of Hepatocellular Carcinoma. Immunotherapy of Hepatocellular Carcinoma 103 119 .
Yi-Fan Zhang & Mitchell Ho. (2016) Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific Reports 6:1.
Crossref
Bryan Fleming & Mitchell Ho. (2016) Glypican-3 Targeting Immunotoxins for the Treatment of Liver Cancer. Toxins 8:10, pages 274.
Crossref
Wei Gao, Yongmei Xu, Jian Liu & Mitchell Ho. (2016) Epitope mapping by a Wnt-blocking antibody: evidence of the Wnt binding domain in heparan sulfate. Scientific Reports 6:1.
Crossref
Wei Gao, Heungnam Kim & Mitchell Ho. (2015) Human Monoclonal Antibody Targeting the Heparan Sulfate Chains of Glypican-3 Inhibits HGF-Mediated Migration and Motility of Hepatocellular Carcinoma Cells. PLOS ONE 10:9, pages e0137664.
Crossref
Hirofumi Hanaoka, Tadanobu Nagaya, Kazuhide Sato, Yuko Nakamura, Rira Watanabe, Toshiko Harada, Wei Gao, Mingqian Feng, Yen Phung, Insook Kim, Chang H. Paik, Peter L. Choyke, Mitchell Ho & Hisataka Kobayashi. (2015) Glypican-3 Targeted Human Heavy Chain Antibody as a Drug Carrier for Hepatocellular Carcinoma Therapy. Molecular Pharmaceutics 12:6, pages 2151-2157.
Crossref
Yi-Fan Zhang, Yen Phung, Wei Gao, Seiji Kawa, Raffit Hassan, Ira Pastan & Mitchell Ho. (2015) New High Affinity Monoclonal Antibodies Recognize Non-Overlapping Epitopes On Mesothelin For Monitoring And Treating Mesothelioma. Scientific Reports 5:1.
Crossref
Hirofumi Hanaoka, Takahito Nakajima, Kazuhide Sato, Rira Watanabe, Yen Phung, Wei Gao, Toshiko Harada, Insook Kim, Chang H Paik, Peter L Choyke, Mitchell Ho & Hisataka Kobayashi. (2015) Photoimmunotherapy of hepatocellular carcinoma-targeting Glypican-3 combined with nanosized albumin-bound paclitaxel. Nanomedicine 10:7, pages 1139-1147.
Crossref
Wei Gao, Zhewei Tang, Yi-Fan Zhang, Mingqian Feng, Min Qian, Dimiter S. Dimitrov & Mitchell Ho. (2015) Immunotoxin targeting glypican-3 regresses liver cancer via dual inhibition of Wnt signalling and protein synthesis. Nature Communications 6:1.
Crossref
Huiping Gao, Kesang Li, Hong Tu, Xiaorong Pan, Hua Jiang, Bizhi Shi, Juan Kong, Hongyang Wang, Shengli Yang, Jianren Gu & Zonghai Li. (2014) Development of T Cells Redirected to Glypican-3 for the Treatment of Hepatocellular Carcinoma. Clinical Cancer Research 20:24, pages 6418-6428.
Crossref
Wei Gao, Heungnam Kim, Mingqian Feng, Yen Phung, Charles P. Xavier, Jeffrey S. Rubin & Mitchell Ho. (2014) Inactivation of Wnt signaling by a human antibody that recognizes the heparan sulfate chains of glypican-3 for liver cancer therapy. Hepatology 60:2, pages 576-587.
Crossref
Mingqian Feng & Mitchell Ho. (2014) Glypican-3 antibodies: A new therapeutic target for liver cancer. FEBS Letters 588:2, pages 377-382.
Crossref
Carlos E. Pedreira, Elaine S. Costa, Quentin Lecrevisse, Jacques J.M. van Dongen & Alberto Orfao. (2013) Overview of clinical flow cytometry data analysis: recent advances and future challenges. Trends in Biotechnology 31:7, pages 415-425.
Crossref
Mingqian FengWei GaoRuoqi WangWeizao Chen, Yan-Gao Man, William Douglas Figg, Xin Wei WangDimiter S. Dimitrov & Mitchell Ho. (2013) Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proceedings of the National Academy of Sciences 110:12.
Crossref

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