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Original Articles

2A to the Fore – Research, Technology and Applications

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Pages 223-260 | Published online: 15 Apr 2013

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Marco Chiabudini, Arlette Tais, Ying Zhang, Sachiko Hayashi, Tina Wölfle, Edith Fitzke & Sabine Rospert. (2014) Release Factor eRF3 Mediates Premature Translation Termination on Polylysine-Stalled Ribosomes in . Molecular and Cellular Biology 34:21, pages 4062-4076.
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M. De Giorgi, I. Pelikant-Malecka, A. Sielicka, E. M. Slominska, R. Giovannoni, A. Cinti, M. G. Cerrito, M. Lavitrano & R. T. Smolenski. (2014) Functional Analysis of Expression of Human Ecto-Nucleoside Triphosphate Diphosphohydrolase-1 and/or Ecto-5′-Nucleotidase in Pig Endothelial Cells. Nucleosides, Nucleotides & Nucleic Acids 33:4-6, pages 313-318.
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Articles from other publishers (32)

Michael A Aimino, Jesse Humenik, Michael J Parisi, Juan Carlos Duhart & Timothy J Mosca. (2023) SynLight: a bicistronic strategy for simultaneous active zone and cell labeling in the Drosophila nervous system . G3: Genes, Genomes, Genetics 13:11.
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Shang Jui Tsai, Yiwei Ai, Chenxu Guo & Stephen J. Gould. (2022) Degron tagging of BleoR and other antibiotic-resistance genes selects for higher expression of linked transgenes and improved exosome engineering. Journal of Biological Chemistry 298:5, pages 101846.
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Alexandra R. F. White, Jose A. Mendez, Aashima Khosla & David C. Nelson. (2022) Rapid analysis of strigolactone receptor activity in a Nicotiana benthamiana dwarf14 mutant . Plant Direct 6:3.
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Xuekun Wang & Mario Andrea Marchisio. (2021) Synthetic polycistronic sequences in eukaryotes. Synthetic and Systems Biotechnology 6:4, pages 254-261.
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Juliana G. S. de Lima & Daniel C. F. Lanza. (2021) 2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology. Viruses 13:11, pages 2160.
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Chengjin Ye, Kevin Chiem, Jun-Gyu Park, Jesus A. Silvas, Desarey Morales Vasquez, Julien SourimantMichelle J. LinAlexander L. GreningerRichard K. PlemperJordi B. Torrelles, James J. Kobie, Mark R. Walter, Juan Carlos de la Torre & Luis Martinez-Sobrido. (2021) Analysis of SARS-CoV-2 infection dynamic in vivo using reporter-expressing viruses. Proceedings of the National Academy of Sciences 118:41.
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Giuseppe Scesa, Raffaella Adami & Daniele Bottai. (2021) iPSC Preparation and Epigenetic Memory: Does the Tissue Origin Matter?. Cells 10:6, pages 1470.
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Ye Sol Jeong, Hyung-Keun Ku, Young-Joo Jung, Jae Kwang Kim, Kyoung Bok Lee, Ju-Kon Kim, Sun-Hyung Lim, Dongho Lee & Sun-Hwa Ha. (2021) 2A-linked bi-, tri-, and quad-cistrons for the stepwise biosynthesis of β-carotene, zeaxanthin, and ketocarotenoids in rice endosperm. Metabolic Engineering Communications 12, pages e00166.
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Asha Ann Philip & John Thomas Patton. (2021) Rotavirus as an Expression Platform of Domains of the SARS-CoV-2 Spike Protein. Vaccines 9:5, pages 449.
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Ekram Ahmed Chowdhury, Guy Meno-Tetang, Hsueh Yuan Chang, Shengjia Wu, Hsien Wei Huang, Tanguy Jamier, Jayanth Chandran & Dhaval K. Shah. (2021) Current progress and limitations of AAV mediated delivery of protein therapeutic genes and the importance of developing quantitative pharmacokinetic/pharmacodynamic (PK/PD) models. Advanced Drug Delivery Reviews 170, pages 214-237.
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Vera S. Efimova, Ludmila V. Isaeva, Philipp S. Orekhov, Marine E. Bozdaganyan, Mikhail A. Rubtsov & Ludmila A. Novikova. (2021) Using a viral 2A peptide-based strategy to reconstruct the bovine P450scc steroidogenic system in S. cerevisiae. Journal of Biotechnology 325, pages 186-195.
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Reyes Sanles-Falagan, Biljana Petrovic-Stojanovska & Malcolm F. White. (2020) Facile and scalable expression and purification of transcription factor IIH (TFIIH) core complex. Protein Expression and Purification 174, pages 105660.
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Juliane Röder, Christina Dickmeis & Ulrich Commandeur. (2019) Small, Smaller, Nano: New Applications for Potato Virus X in Nanotechnology. Frontiers in Plant Science 10.
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Krishna Kumar Haridhasapavalan, Manash P. Borgohain, Chandrima Dey, Bitan Saha, Gloria Narayan, Sachin Kumar & Rajkumar P. Thummer. (2019) An insight into non-integrative gene delivery approaches to generate transgene-free induced pluripotent stem cells. Gene 686, pages 146-159.
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Anja Hofmann, Johannes Falk, Tim Prangemeier, Dominic Happel, Adrian Köber, Andreas Christmann, Heinz Koeppl & Harald Kolmar. (2019) A tightly regulated and adjustable CRISPR-dCas9 based AND gate in yeast. Nucleic Acids Research 47:1, pages 509-520.
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Alexandra Merkx-Jacques, Holly Rasmussen, Denise M. Muise, Jeremy J. R. Benjamin, Haila Kottwitz, Kaitlyn Tanner, Michael T. Milway, Laura M. Purdue, Mark A. Scaife, Roberto E. Armenta & David L. Woodhall. (2018) Engineering xylose metabolism in thraustochytrid T18. Biotechnology for Biofuels 11:1.
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Tom Schreiber & Alain Tissier. 2018. Molecular Pharming. Molecular Pharming 25 42 .
Stanislava Gunišová, Vladislava Hronová, Mahabub Pasha Mohammad, Alan G Hinnebusch & Leoš Shivaya Valášek. (2017) Please do not recycle! Translation reinitiation in microbes and higher eukaryotes. FEMS Microbiology Reviews 42:2, pages 165-192.
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Juliane Röder, Christina Dickmeis, Rainer Fischer & Ulrich Commandeur. (2018) Systemic Infection of Nicotiana benthamiana with Potato virus X Nanoparticles Presenting a Fluorescent iLOV Polypeptide Fused Directly to the Coat Protein . BioMed Research International 2018, pages 1-12.
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Garry A. Luke & Martin D. Ryan. 2018. Reporter Gene Assays. Reporter Gene Assays 31 48 .
Gary Loughran, Michael T. Howard, Andrew E. Firth & John F. Atkins. (2017) Avoidance of reporter assay distortions from fused dual reporters. RNA 23:8, pages 1285-1289.
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Juliane Röder, Rainer Fischer & Ulrich Commandeur. (2017) Adoption of the 2A Ribosomal Skip Principle to Tobacco Mosaic Virus for Peptide Display. Frontiers in Plant Science 8.
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Julius Grzeschik, Steffen C. Hinz, Doreen Könning, Thomas Pirzer, Stefan Becker, Stefan Zielonka & Harald Kolmar. (2017) A simplified procedure for antibody engineering by yeast surface display: Coupling display levels and target binding by ribosomal skipping. Biotechnology Journal 12:2, pages 1600454.
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Deborah NaonMarta ZaninelloMarta GiacomelloTatiana VaranitaFrancesca GrespiSowmya LakshminaranayanAnnalisa SerafiniMartina SemenzatoStephanie HerkenneMaria Isabel Hernández-AlvarezAntonio ZorzanoDiego De StefaniGerald W. DornIIII & Luca Scorrano. (2016) Critical reappraisal confirms that Mitofusin 2 is an endoplasmic reticulum–mitochondria tether. Proceedings of the National Academy of Sciences 113:40, pages 11249-11254.
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Sangram K. Lenka, Nicole Carbonaro, Rudolph Park, Stephen M. Miller, Ian Thorpe & Yantao Li. (2016) Current advances in molecular, biochemical, and computational modeling analysis of microalgal triacylglycerol biosynthesis. Biotechnology Advances 34:5, pages 1046-1063.
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Sivasundaram Karnan, Akinobu Ota, Yuko Konishi, Md Wahiduzzaman, Yoshitaka Hosokawa & Hiroyuki Konishi. (2016) Improved methods of AAV-mediated gene targeting for human cell lines using ribosome-skipping 2A peptide. Nucleic Acids Research 44:6, pages e54-e54.
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Juliana de Amorim Araújo, Túlio César Ferreira, Marciano Régis Rubini, Ana Gilhema Gomez Duran, Janice Lisboa De Marco, Lidia Maria Pepe de Moraes & Fernando Araripe Gonçalves Torres. (2015) Coexpression of cellulases in Pichia pastoris as a self-processing protein fusion. AMB Express 5:1.
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Ákos Boros, Péter Pankovics & Gábor Reuter. (2014) Avian picornaviruses: Molecular evolution, genome diversity and unusual genome features of a rapidly expanding group of viruses in birds. Infection, Genetics and Evolution 28, pages 151-166.
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Qichao Zhao, Minghong Liu, Miaomiao Tan, Jianhua Gao & Zhicheng Shen. (2014) Expression of Cry1Ab and Cry2Ab by a Polycistronic Transgene with a Self-Cleavage Peptide in Rice. PLoS ONE 9:10, pages e110006.
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F Alaee, O Sugiyama, M S Virk, H Tang, H Drissi, A C Lichtler & J R Lieberman. (2013) Suicide gene approach using a dual-expression lentiviral vector to enhance the safety of ex vivo gene therapy for bone repair. Gene Therapy 21:2, pages 139-147.
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Heather Wanczyk, Tolga Barker, Debra Rood, Daniel Zapata, Amy Howell, Stewart Richardson, John Zinckgraf, Gregory Marusov, Michael Lynes & Lawrence Silbart. (2013) Cloning and Characterization of a Hybridoma Secreting a 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-Specific Monoclonal Antibody and Recombinant F(ab). Toxins 5:3, pages 568-589.
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Frank Sainsbury, Meriem Benchabane, Marie-Claire Goulet & Dominique Michaud. (2012) Multimodal Protein Constructs for Herbivore Insect Control. Toxins 4:6, pages 455-475.
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