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Immunoassay

Novel Enzyme-Linked Aptamer Assay for the Determination of Aflatoxin B1 in Peanuts

ORCID Icon, , &
Pages 2961-2973 | Received 18 Apr 2019, Accepted 12 Jun 2019, Published online: 26 Jun 2019
 

Abstract

A novel enzyme-linked aptamer assay is reported for the determination of aflatoxin B1 (AFB1). AFB1 can competitively bind with the immobilized biotin-aptamer and release biotin complementary DNA, leading to the gradual fading of the detection system color with increasing of AFB1 concentration. In the absence of AFB1, the biotinylated complementary DNA is not be released from the fixed aptamer. Therefore, the enzyme reaction occurs in the detection system. Under the optimized experimental conditions, the proposed method possessed a wide linear range for AFB1 from 1 to 80 ng/mL (R2 of 0.990) with a low detection limit of 0.36 ng/mL. The method was then applied to detect uncontaminated peanuts fortified with different concentrations of AFB1. The recovery values were from 82.60% to 94.43%, which indicated the proposed method may be used to detect AFB1 in food and has potential for the development of test kits.

Disclosure statement

The authors declare that they have no competing interests.

Additional information

Funding

This work was supported by- Key Research and Development and Promotion Project of the Science and Technology Department of Henan Province [grant number 192102310254] and the Emerging Industry Research Project of Zhengzhou City, China [grant number 20150503].

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