On-Site Determination of Classical Swine Fever Virus (CSFV) by a Fluorescent Microsphere-Based Lateral Flow Immunoassay Strip (FM-LFIAs) Based on Monoclonal Antibodies

Abstract

Classical swine fever (CSF) is a highly infectious and lethal multisystem disease caused by the classical swine fever virus (CSFV). This virus has resulted in huge economic losses in the porcine industry. Rapid detection of CSFV is needed in order to prevent and control the early transmission of CSFV. In this study, nine hybridoma cell lines that secrete monoclonal antibodies against CSFV (anti-CSFV-mAb) were prepared using the cell fusion and cell surface fluorescent immunosorbent assay (CSFIA). A rapid, sensitive, and specific fluorescent microsphere-based lateral flow immunoassay strip (FM-LFIAs) based on the use of paired CSFV-mAbs was developed. The sensitivity of the FM-LFIAs for CSFV detection was 5.28 ng/mL with a linear range from 9.77 to 625 ng/mL. During clinical sample assays, the FM-LFIAs results were consistent with the measurements obtained using the reverse transcription – polymerase chain reaction (RT-PCR), highlighting the practical application of FM-LFIAs for the on-site detection of CSFV.

Keywords:

• Cell surface fluorescence immunosorbent assay
• Classic swine fever virus
• Fluorescent microsphere-based lateral flow immunoassay strip
• Monoclonal antibody

Disclosure statement

The authorss declare that they have no competing interests.

Additional information

Funding

This work was supported by the National Key Research and Development Program of China (2016YFD0500600).