Characterization of the Noncovalent Interactions between Lysozyme and Panaxadiol Glycosides by Intensity-Fading – Matrix-Assisted Laser Desorption Ionization – Mass Spectrometry (IF-MALDI-MS)

Abstract

The interactions of lysozyme with three panaxadiol glycosides (Rd, Rg3, Rh2) were investigated using intensity-fading – matrix-assisted laser desorption ionization – mass spectrometry (IF-MALDI-MS). The results showed that the noncovalent interactions of lysozyme with panaxadiol glycosides were recognized by IF-MALDI-MS. The affinities of three glycosides toward lysozyme were assessed. This strategy provides a rapid and sensitive approach to investigate the interactions of lysozyme with glycosides by IF-MALDI-MS that provides the insights of mechanisms of glycosides and also extends its application.

Keywords:

• Intensity-fading – matrix-assisted laser desorption ionization – mass spectrometry (IF-MALDI-MS)
• panaxadiol glycosides
• protein interactions

Disclosure statement

The authors declare that they have no conflicts of interest.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China by grant number 21675150.