Determination of Acetamiprid by Fluorescence Monitoring of a Glycine-L-Histidine Copper-Organic Framework Aptasensor

Abstract

The sensitive determination of pesticides constitutes an important aspect in ensuring food safety. Due to the disadvantages of traditional methods, such as low sensitivity, complex operation, and high cost, the development of an efficient and economical biosensor to detect pesticide residues is required. In this work, the dipeptide glycyl-L-histidine (GH) and divalent copper ions (Cu²⁺) were employed as bioorganic linkers and metal ions in the synthesis of the peptide-based metal organic framework (MOF) material GH-Cu BioMOF. Because of its properties of fluorescence quenching and adsorption of nucleic acids, GH-Cu BioMOF was used to develop a distinctive aptamer based method for the determination of pesticide residues in food. The GH-Cu BioMOF-based aptasensor was used for acetamiprid and demonstrated a linear response in the range from 0.15 to 3.00 µM with a detection limit of 137 nM, verifying the feasibility of this method for pesticide determination in food.

Keywords:

• Metal organic framework
• aptamer sensor
• acetamiprid
• fluorescence
• glycine-L-histidine

Additional information

Funding

This work was financially supported by the National Key Research and Development Project of China (grant 2019YFC1605305), Hunan Provincial Natural Science Foundation of China (grant 2019JJ50998), National Natural Science Foundation of China (grant 31972173), Scientific Research Fund of Hunan Provincial Education Department (grant 18B165), and Program for Science and Technology Innovation Platform/Talents of Hunan Province (grant 2019TP1029, 2018XK2006).