![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A simple and facile hydrothermal method was developed for the synthesis of gold nanoparticles (AuNPs) adorned with two-dimensional (2D) heme-like Fe(III)-tetra(4-carboxyphenyl)-porphine (TCPP-Fe) metal-organic framework (MOF) nanocomposite. Notably, the synthesized hybrid nanomaterial (AuNPs/TCPP-Fe hybrid nanosheets) exhibited excellent peroxidase (POD)-like nanoenzymes activity and Raman activity under ultrasound (US) and near-infrared (NIR) irradiation at physiological pH conditions. A cascade colorimetric-SERS dual-mode method was developed for sensitive and rapid cholesterol determination based on the excellent POD-like properties and surface-enhanced Raman spectroscopy (SERS) activity of AuNPs/TCPP-Fe nanoenzymes. The TCPP-Fe nanoenzymes combined with cholesterol oxidase catalyze a cascade reaction to convert cholesterol to H2O2, which then oxidizes colorless, low Raman-activity 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue, high-Raman-activity oxidation product (ox-TMB). The cholesterol in human serum of colorimetric-SERS was successfully assayed with good recovery from 97.8 to 103.7%. The study paves a new method for designing a nanocomposite-based SERS for biochemistry analysis.
Acknowledgements
This work was supported by Analysis and Testing Foundation of Kunming University of Science and Technology (No. 2021T20200097).
Ethical approval
This study was approved by the Kunming University of Science and Technology Ethic Committee and performed in accordance with the Guidelines for Experimentation of Kunming University of Science and Technology.
Informed consent
The blood samples were collected from volunteers who provided informed written consent for inclusion.
Disclosure statement
No potential conflict of interest was reported by the authors.