EDACO, a derivative of myricetin, inhibits the differentiation of Gaoyou duck embryonic osteoclasts in vitro

ABSTRACT

1. This study determined the effects of (E)-3-(2-(4-(3-(2,4-dimethoxyphenyl)acryloyl)phenoxy)ethoxy)-5,7-dimethoxy-2-(3,4,5-trimethoxyphenyl)-4H-chromen-4-one (EDACO) on the differentiation of Gaoyou duck embryonic osteoclasts cultured in vitro.

2. Bone marrow mononuclear cells (BM-MNC) were collected from 23-d-old Gaoyou duck embryos and induced by macrophage colony-stimulating factor and receptor activator of nuclear factor κB ligand in the presence of EDACO at different concentrations (i.e. 10, 20, 40, 80 and 160 µM). Tartrate-resistant acid phosphatase (TRAP) staining and resorption ability determination were conducted.

3. Results suggested that EDACO suppressed the shaping of positive multinucleated cells and the number of TRAP-positive cells in the 20, 40, 80 and 160 μM EDACO groups was significantly decreased (P < 0.05 or P < 0.01). Besides, the absorption activity of differentiated duck embryonic osteoclasts was significantly inhibited (P < 0.05) in both 80 and 160 μM EDACO groups.

4. Overall, EDACO can inhibit the differentiation of BM-MNC into mature osteoclasts in duck embryos.1

KEYWORDS:

• Biochemistry
• bone biology
• cell biology
• ducks
• embryology
• osteoporosis
• pharmacology

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported by the National Nature Science Foundation of China [No. 21462012], the Special Fund for Outstanding Scientific and Technological Candidates of Guizhou Province [No. 201535], the Project of Natural Science Foundation of Anhui Province [1508085QH172], the Natural Science Research Project of Universities in Anhui [KJ2017A225, KJ2017A237, KJ2018A1011], the Natural Science Foundation of Bengbu Medical College [BYKY1614ZD, BYKF18107] and the National Training Programs of Innovation and Entrepreneurship for Undergraduate [201410367029, 201610367004, 201810367019].