Identification of differentially expressed genes and signalling pathways in the ovary of higher and lower laying ducks

ABSTRACT

1. Ovarian transcriptomic profiling between birds showing high egg number (HEN) and birds of low egg number (LEN) in Longyan Shan-ma ducks at 71 weeks of age was carried out using Illumina Hiseq 2500 technology.

2. A total of 343 differentially expressed genes (DEGs, 269 upregulated and 74 downregulated) were identified between HEN and LEN ovaries. These DEGs were enriched in 30 Gene Ontology terms. Pathway functional analysis found that the DEGs were enriched in 10 metabolic pathways (P < 0.05), one of which was regulation of the actin cytoskeleton pathway (Q < 0.05).

3. Three integrin family genes, ITGB2, ITGB5 and ITGA8 were differentially expressed in the RNA-seq and qPCR experiments.

4. The DEGs and signalling pathways identified in ovarian tissue in this study provide new insights into high egg production in Longyan Shan-ma duck.

KEYWORDS:

• Duck
• egg production
• RNA-seq
• signalling pathways
• integrin beta family genes

Acknowledgments

This study was supported by the following grants: Key Research and Breeding Projects and Industrialization Foundations of Agricultural Improved Breeding in Breed Industry Innovation and Industrialization Project (2017-2020) of Fujian Province (fjzycxny2017011); Science and Technology Department Leading Project of Fujian Province (2018N0021); Agricultural Projects Foundation of Longyan Science and Technology Bureau (2019LYF1002 and 2019LYF12002); Longyan University Scientific Research Fund for the Doctoral Young Scholars (LB9001). We thank the International Science Editing (http://www.internationalscienceediting.com) for editing this manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

Supplemental data for this article can be accessed here.

Additional information

Funding

This study was supported by the following grants: Key Research and Breeding Projects and Industrialization Foundations of Agricultural Improved Breeding in Breed Industry Innovation and Industrialization Project (2017-2020) of Fujian Province (fjzycxny2017011); Science and Technology Department Leading Project of Fujian Province (2018N0021); Agricultural Projects Foundation of Longyan Science and Technology Bureau (2019LYF1002 and 2019LYF12002); Longyan University Scientific Research Fund for the Doctoral Young Scholars (LB9001). We thank International Science Editing (http://www.internationalscienceediting.com) for editing this manuscript.