Flowering and morphological characterization of Dendrocalamus asper androecium and pollen grains

Abstract

Little is known about the reproduction of Dendrocalamus asper because it flowers only every 100 to 120 years. In the present work we describe some reproductive features of this bamboo and characterise flowers and pollen at various developmental stages. Number of pollen grains and ovules per flower, pollen/ovule ratio, in vitro twinning and pollen grain viability in vivo were evaluated and the different stages of floral development identified. Further, we performed a morphological analysis of androecium and pollen development. Seven distinct stages of flower development were identified; four initial stages, a pre-anthetic stage, and two stages of anthetic. Dendrocalamus asper pseudospikelets avoid inbreeding by means of protogyny. The floral and pollen characteristics suggest that the species is anemophilous. The ultrastructural characteristics of free microspores (stage two of floral development), vacuolated microspores (stage five) and mature pollen (anthetic) were analysed. During maturation, pollen grains accumulate larger and more numerous amyloplasts and organelles such as mitochondria. Pollen disperse in the tricellular development stage. Pollen is monoporate with an operculum-like pore, with a rugulate structure and a spinose tectum.

Keywords:

• bamboo
• wind-pollination
• microgametogenesis
• reproductive biology

Acknowledgements

The authors also thank the Jatobás Institute for the donation of plant material.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the National Council for Scientific and Technological Development (CNPq) [457726/2013-0]; Rosete Pescador [302105/2017-4]; Miguel Pedro Guerra [302798/2018-8]; the Coordination for the Improvement of Higher Education Personnel (CAPES) for the doctoral level scholarship to Priscila Fernandes de Souza.