Mechanical characteristics of BMSCs-intervened sciatic nerve in chronic alcohol-intoxicated animal model

Abstract

Objective

To study the mechanical properties of sciatic nerve in rats with chronic alcoholism (CA) and intervened with bone marrow mesenchymal stem cells (BMSCs) and to provide biomechanical basis for clinical practice.

Methods

the serum of the BMSCs-intervened CA rats was sampled and determined the contents of malondialdehyde (MDA), metallothionein (CAS, MT), and Glutathione/r -glutamyl cysteinyl/glycine (GSH); meanwhile, the rats’ sciatic nerve was tested the tensile and observed the histomorphological changes.

Results

The mechanical properties of sciatic nerve in BMSCs-intervened CA rats, as well as the serum levels of MT and GSH, were significantly different from those in the basic fibroblast growth factor (bFGF)-intervened CA rats (p < 0.05).

Conclusions

BMSCs intervention can restore the levels of MT, GSH, MDA, histomorphology, and tensile mechanical properties in CA animal model, and its effects on repairing sciatic nerve are obvious.

Keywords:

• Animal model of alcoholism
• sciatic nerve injury
• bone marrow mesenchymal stem cell intervention
• basic fibroblast growth factor intervention
• mechanical property

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This study was funded by the Projects supported by Jilin Provincial Science and Technology Development Plans (2016c041-3).