Storage conditions significantly influence the stability of stingless bee (Melipona scutellaris) honey

Abstract

Stingless bees are endemic in northeastern Brazil, where they are exploited for honey production and crop pollination. The therapeutic use of honey can be attributed to its antibacterial and antioxidant activities. The effects of time and storage temperature on Melipona scutellaris honey were evaluated by physicochemical and microbiological parameters as well as by the analysis of bioactive compounds and antioxidant activity. Fresh honey samples were monitored at 5 and 30 °C for 150 days. The total acidity and HMF increased, while pH and diastase activity significantly decreased over time at 30 °C (p < 0.05). Fresh honey met all the established parameters. In contrast, due to storage, it failed to meet the criteria set in the current legislation for Melipona with the most drastic shortcomings observed at 30 °C. Unlike monosaccharides, acetic and lactic acid concentrations increased, indicating fermentation. During storage, the total phenolic compounds and flavonoids contents were reduced along with antioxidant activities measured by DPPH, ABTS, and FRAP methods. On the 60th day of storage at 5 °C, dextrose crystallization began, and the water content increased. Storage at 5 °C for up to 60 days resulted in minor changes, but after 150 days, storage at 30 °C showed reduced bioactive degradation and higher antioxidant activity. Therefore, the storage conditions associated with crystallization were found to control the stability of Melipona scutellaris honey.

Keywords:

• regulation
• hydroxymethylfurfural (HMF)
• antioxidant activity
• time
• temperature
• crystallization

Acknowledgements

The authors are grateful for the scholarship FAPESB BOL 4874/2017 granted to Larissa Farias da Silva Cruz, to Mr. Pedro Viana (Meliponary Costa do Saiupe – BA) for the honey samples, to professors Carolina Oliveira de Souza, Ph.D., and Janice Izabel Druzian, Ph.D. National Council of Technological and Scientific Development (CNPq) productivity fellows 313641/2019-6, and 311392/2016-4.

Disclosure statement

The authors report no conflicts of interest in this work.

Supplementary material

Supplementary Tables 1–5 are available via the ‘Supplementary’ tab on the article’s online page (http://dx.doi.org/10.1080/00218839.2021.1950971).

Additional information

Funding

This work was funded by Fundação de Amparo à Pesquisa do Estado da Bahia; Conselho Nacional de Desenvolvimento Científico e Tecnológico; Conselho Nacional de Desenvolvimento Científico e Tecnológico.