Preparation of Injectable Sodium Alginate/Platelet Rich Plasma Porous Microcarriers

Abstract

Porous sodium alginate/platelet rich plasma (SA/PRP) microcarriers were fabricated using an emulsion method combined with a freeze-drying method. The effects of the composition, concentration and freeze temperature on the properties of the porous microcarriers were studied. The optimum conditions for fabrication of the SA/PRP microcarriers were determined as −20 °C, SA concentration of 2.0 wt% and mass ratio of 2:1 (SA to PRP) for carrying cells. The SA/PRP microcarriers, with an average diameter of 100–350 μm, showed an interconnective porous structure and the pore size was about 30–70 μm. Such porous SA/PRP may be considered as a promising candidate for injectable carriers supporting attachment and proliferation of cells.

Keywords:

• Sodium alginate
• platelet rich plasma
• porous microcarriers

Additional information

Funding

This work was financially supported by Scientific Research Fund of Hunan Provincial Education Department (No. 21A0323) and National Experimental Teaching Demonstration Center of Chemical Engineering and Materials.