Cytological comparison of mycelial aggregates of Rhizopogon roseolus with and without the ectomycorrhiza host

ABSTRACT

Most cytology reports on ectomycorrhiza (ECM) have been less focused on the fungal structures, especially on the exploratory organ. This study aimed to evaluate the morphological response of explorative mycelia of ECM forming fungi with and without the occurrence of the ECM host. We assessed the mycelial aggregates of Rhizopogon roseolus which was inoculated with and without Pinus thunbergii under controlled laboratory conditions. The mycelial aggregates with the host produced strikingly complex hyphal strands relative to those without host. Light microscopy revealed that the cytology and plectology of both mycelial aggregates had the approximately similar architecture. The tubular hyphae diameter without the host was consistently larger than that with the host. This study confirmed that the septa diameter of the tubular hyphae conjunction of mycelial aggregates with the host were shorter than those without the host. In addition, partially and completely dissolved septa of the tubular hyphae were evident with and without host. We also described the thromboplerous hyphae, which have rarely been reported in vitro. These hyphae were produced in higher numbers near the fungal inoculum with and without the host. The current findings will add to the current knowledge regarding ECM fungi mycelial aggregate response to the host.

KEYWORDS:

• Assessment
• mycelial aggregates
• Rhizopogon roseolus
• with/without host

ACKNOWLEDGMENTS

The authors are grateful to Editage (www.editage.com) for English language editing of the manuscript.

DISCLOSURE STATEMENT

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant number 21K05710.