Comparison of a Chilean strain of the ichthyotoxic phytoflagellate Heterosigma akashiwo (Raphidophyceae) with strains from France, Spain and New Zealand

ABSTRACT

Heterosigma akashiwo is a bloom-forming planktonic flagellate that causes fish mortalities, which damages the aquaculture industry worldwide. In Chile, this species was reported for the first time in 1988 when it caused one of the most dramatic harmful algal blooms in the south of the country. At that time, an unialgal culture was set and used for a morphological characterization but, unfortunately, it was not maintained. In April 2013 a new outbreak of H. akashiwo was detected in the Maullín Estuary (41°37′S, 73°36′W), Southern Chile, causing fish mortalities in commercial salmon farms. An unialgal culture of this new Chilean population of H. akashiwo was obtained and has been maintained in the Culture Collection of Microalgae of the University of Concepción, Chile, under the code CCM-UdeC 225. The aim of this study was to compare the morphological and physiological attributes of this Chilean strain of H. akashiwo with those of strains from France, Spain and New Zealand, in order to identify eventual intraspecific variability among them. Cell size, growth parameters, pigments profiles, photosynthetic attributes and toxicity were determined for the strains, grown under identical culture conditions. The Chilean strain exhibited the highest growth and accumulated the highest proportion of the carotenoid fucoxanthin, while strains from Chile and Spain were the less light-tolerant and had similar toxicity. These results reinforce previous findings about the high level of intraspecific variability within H. akashiwo including, for the first time, a strain from South America.

KEYWORDS:

• Fish-killing microalgae
• Intraspecific variability
• South American strain

ACKNOWLEDGEMENTS

This work was supported by the COPAS-Sur Austral Program (CONICYT Grant PFB-31) Universidad de Concepción, Chile and Consortium Algaefuels, grant 09CTEI6861-04. We thank Miriam Seguel (CERAM-Universidad Austral de Chile), who kindly donate the strain HA94CAM (France), Victor González (Universidad de Concepción) for technical support in pigment quantification, and Dr. Mariela González S. (Universidad de Concepción) for helpful comments to the manuscript.

DISCLOSURE STATEMENT

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the COPAS-Sur Austral Program [CONICYT Grant PFB-31]; Universidad de Concepción, Chile and Consortium Algaefuels [09CTEI6861-04].