Evaluation of the Atellica^® UAS 800: a new member of the automated urine sediment analyzer family

Abstract

Background

In 2017 the Atellica^® UAS 800 urine sediment analyzer was introduced by Siemens Healthineers. We investigated its applicability in the standardization and automation of the laboratory urinalysis workflow, including the prediction of urine culture outcome and glomerular pathology.

Methods

We evaluated the performance characteristics of the Atellica^® UAS 800 and its correlation with the iQ200 (Beckman Coulter). In addition, we studied the agreement between Atellica^® UAS 800 and CLINITEK Novus^® and determined the predictive value of bacteria and leukocyte counts for urine culture outcome. Furthermore, we investigated the ability of Atellica^® UAS 800 to identify pathological casts and dysmorphic erythrocytes in comparison to manual microscopy.

Results

Erythrocyte and leukocyte analyses indicated high intra- and inter-run precisions and good correlations with the iQ200. We found that the Atellica^® UAS 800 detects bacteria with higher sensitivity than the iQ200. The Atellica^® UAS 800 and CLINITEK Novus^® showed a high degree of conformity. We determined seven combinations of clinical cut-off values of bacteria and leukocytes for predicting urine culture outcome with sensitivity, specificity, and negative predictive values of 95%, 52%, and 93%, respectively. Using the Atellica^® UAS 800, hyaline casts, erythrocyte casts, leukocyte casts, and dysmorphic erythrocytes were correctly recognized in 76%, 22%, 2%, and 39% of the samples, respectively.

Conclusions

The Atellica^® UAS 800 is a robust, fast, and user-friendly analyzer, which accurately quantifies erythrocytes, leukocytes, bacteria and squamous epithelial cells, and may be utilized for predicting positive urine cultures. The detection of clinically important pathological casts and dysmorphic erythrocytes proved insufficient.

Keywords:

• Laboratory automation
• urinalysis
• hematuria
• validation study
• urinary tract infections

Acknowledgements

The authors thank the technicians of the MMC and UMCU laboratories who were involved in preparing, shipping and analyzing the urine samples from this study.

Ethical approval

The study was approved by the Institutional Scientific Research Review Committee of Meander Medical Centre (study number TWO 20-068), including data- and material transfer agreements.

Disclosure statement

No potential conflict of interest was reported by the author(s).