Protein expression by bone mesenchymal stem cells cultivated in PLLA scaffolds with different pore geometry

Abstract

The effects of poly(L,L-lactide) (PLLA) scaffold with axial and isotropic structure were investigated on functional activity of rabbit bone mesenchymal stem cells (BMSCs). PLLA scaffolds were processed by freeze-dry technique at different temperatures of the scaffold frost – −196 °C, −25 °C and 0 °C. Scaffolds with different pore sizes were obtained by adding 5 or 10% of water phase. Scaffolds were modified by collagen type I solution. The pore sizes of polymer scaffolds were ranging from 5 to 150 µm. More protein secretion was observed in the surface-modified scaffolds than in the unmodified after 2 weeks of cultivation in vitro.

Graphical Abstract

KEYWORDS:

• Bone marrow mesenchymal stromal cells
• extracellular proteins
• poly(L,L-lactide) scaffolds
• pore geometry
• porosity

Disclosure statement

The authors have no conflicts of interest to report in this work.

Additional information

Funding

The cell cultivation and analysis study was supported by a grant from the Russian Science Foundation [No. 14-50-00068] and with financial support from the Federal Agency of Scientific Organizations (Russia). The mechanical testing study was supported by a grant from the Russian Science Foundation [No. 14-33-00003]. The authors are grateful to Alyona Kashirova for help to determine relative porosity.