Abstract
The encapsulation of single T cells has gained interest for cell-based therapy. We aimed to examine the biological activity of encapsulated cells after preservation under different conditions which were preservation at −80 °C and cryopreservation. Jurkat cells were encapsulated in polyelectrolytes (PAH or PSS) and in the layer of polyelectrolyte-coated gold nanorods (GNR-PSS). The encapsulation layer and the gold content could be detected after both types of preservation. The viability of encapsulated preserved Jurkat cells were in the range of ∼80–90%. TNF-α secretion was not induced. However, a significant induction of IL-6 levels was detected in encapsulated cells stored at −80 °C.
Graphical Abstract
Acknowledgements
We thank the Center of Excellence on Environmental Health and Toxicology, the Center of Nanoimaging, Toxicology Program, and Materials Science and Engineering Program, Faculty of Science, Mahidol University for kindly providing the facilities used for this research.
Disclosure statement
The authors declare no conflict of interest.