A comparative study of monoaxial and coaxial PCL/gelatin/Poloxamer 188 scaffolds for bone tissue engineering

Abstract

Structure of fibrous scaffolds varies depending on the electrospinning method and parameters which will define cell fate. In this study, monoaxial and coaxial electrospun PCL/gelatin/P-188 scaffolds were characterized in terms of morphology, chemistry, mechanical property, water retention, weight loss, porosity and release properties. Coaxial fibers supported duel release of model hydrophilic protein (Beta-lactoglobulin) and hydrophobic agent vitamin K2. Novel PCL/P-188(Shell):Gelatin/P-188(Core) coaxial scaffold had superior stability, average weight loss, no swelling behavior, controlled release of Beta-lactoglobulin and vitamin K2, enhanced Saos-2 cell viability and ALP activity and could be a good candidate as a bone tissue scaffold.

Graphical Abstract

Keywords:

• Wet coaxial electrospinning
• poloxamer 188
• dual release
• bone scaffold
• beta-lactoglobulin
• vitamin K2