Abstract
In this paper, gelatin methacrylate hydrogel scaffold, functionalized with growth factors loaded alginate-chitosan core-shell nanoparticles, were developed to enhance chondrogenic differentiation of adipose-derived mesenchymal stem cells. With the chosen preparation conditions, the encapsulation efficiency of transforming growth factorβ-1 (TGFβ-1) and insulin-like growth factor-1 (IGF-1) was calculated at 93.34% and 91.92%, respectively. The assay groups which contain loaded nanoparticles in hydrogels exhibited the highest DNA and glycosaminoglycan contents with 2.93 and 573.60 µg/sample, respectively. Moreover, in the same group, it was observed that the amount of collagen also increased by 3.6-fold. Thus, the functionalized scaffold is promising construct for tissue engineering applications.
Graphical Abstract
![](/cms/asset/b6e15aa2-2981-4f3d-a782-6ef77c6bbb90/gpom_a_2014484_uf0001_c.jpg)
Acknowledgments
The authors thank Pezhman Hosseinian for his valuable contributions to the synthesis of GelMA.
Disclosure statement
The authors have declared that there is no conflict of interest.