Design of oxoperoxovanadium(V) complexes and their DNA interaction studies

Abstract

Two oxoperoxovanadium(V) complexes, [VO(O₂)(BHA)(bp)] (1) and [VO(O₂)(BHA)(phen)] (2), containing benzhydroxamic acid (BHA), 1,10-phenanthroline (phen) and 2,2’-bipyridyl (bp) ligands are synthesized and the structure of the complexes have been confirmed by single-crystal X-ray crystallography. The DNA-binding studies of the complexes as assessed by UV absorption and fluorescence methods using CT-DNA suggest the possible mode of interaction as groove binding. The gel electrophoresis further supports the feasible interactions of complexes with DNA. The binding constants (K_b) and Stern-Volmer (K_SV) constants of 1 and 2 are 5.4(±0.21)×10⁴ M⁻¹ and 5.1(±0.10)×10⁴ M⁻¹ and 5.668(±0.08)×10⁵ M⁻¹ and 1.468(±0.07)×10⁵ M⁻¹, respectively.

Graphical Abstract

Keywords:

• Oxoperoxovanadium(V)
• benzhydroxamic acid
• 1,10-phenanthroline
• 2,2’-bipyridyl
• DNA-binding

Acknowledgements

The authors thank Vellore Institute of Technology, Vellore for providing necessary facilities and SAS-VIT-SIF for the UV, IR and fluorescence spectrophotometer. Thanks are also due to ‘VIT SEED GRANT’. The authors are sincerely thankful to Dr. R. Vijayaraghavan, SAS, VIT for the support and guidance. Thanks are also due to Dr. Priyankar Paira, SAS, VIT for supporting us in biological studies and Dr. T.B. Sridharan, SBST, VIT for having helped us in carrying out gel electrophoresis studies.