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Abstract
Aim
The aim of the study was to assess the toxicity profile of Selaginella bryopteris extract and evaluate its wound healing activity.
Methods
In vitro wound healing activity of S. bryopteris extract (5% and 10%) was performed using Clonogenic and Scratch assays. The toxicity profile of S. bryopteris extract ointment was evaluated on animals using acute toxicity and dermal toxicity tests. In vivo wound healing activity of S. bryopteris extract ointment (5% and 10%) was used to determine tensile strength in the incision wound healing model.
Results
Results exhibited that the extract was safe up to 2000 mg/kg per oral dose and non-reactive while applied topically. In vitro results showed that S. bryopteris extract closed the wound gap created by 97.13% in 48 h. The clonogenic assay revealed that the surviving factor for HaCaT cells and MEF cells was 0.78 and 0.85 after treated with 10% concentrations of S. bryopteris. The tensile strength exhibited by S. bryopteris 5% and 10% groups was 395.4 g and 558.5 g in comparison to the control group.
Conclusion
Thus, S. bryopteris extract can be used as an alternative safe drug therapy against topical wounds.
Acknowledgement
The authors are thankful to our Honorable Director, CSIR-NBRI, Lucknow, for their assistance and for providing laboratory facilities. I am so much thankful to my colleague Mrs. Pritt Verma (SRF) for their constant support during the experiment and Animal work. Further, we extend our gratitude to the University Grant Commission-RGNSRF, New Delhi, India, for providing a grant to perform the study (Grant Number F1-17.1/2014–15-RGNF-2014–15-SC-UTT-60684).
Disclosure statement
The authors declared no conflict of interest.