Aqueous two-phase extraction of punicalagin (α+β) from pomegranate peel by response surface methodology

ABSTRACT

In order to enhance the recovery of punicalagin from pomegranate peel, extraction of punicalagin (α + β) was studied using aqueous two-phase system. Box Behnken design of response surface methodology was used as a tool to study the effect of Poly Ethylene Glycol (PEG) concentration, PEG molecular weight, salt concentration, solute amount and pH on the extraction yield of punicalagin. The optimal conditions were found to be 18% (w/v) PEG concentration, 8000 PEG molecular weight, 16% (w/v) salt concentration, 0.5 g solute amount and 6 pH. Under these conditions, experimental yield and predicted yield was found to be 43% and 48%, respectively.

KEYWORDS:

• Punicalagin (α+β)
• pomegranate peel
• Punica gratinum
• aqueous two phase systems
• polyethylene glycol
• potassium dihydrogen orthophosphate
• Box Behnken