Combination of a deep eutectic solvent and macroporous resin for green recovery of anthocyanins from Nitraria tangutorun Bobr. fruit

ABSTRACT

This study aimed at extracting and separating anthocyanins from Nitraria tangutorun Bobr. fruit using deep eutectic solvent (DES) combined with macroporous resins. Choline chloride/1,2-propanediol (ChCl-Prop)-based DESs produced higher extraction yields of total anthocyanins from N. tangutorun fruit than pure water and ethanol/methanol/acetone aqueous solutions did, and LX-32 macroporous resin was used to separate total anthocyanins from the DES (recovery＞95%). Additionally, a green and efficient HPLC-DAD method was used for the analysis of total anthocyanins using ethanol and a tartaric acid aqueous solution as mobile phases, and 13 anthocyanins were identified from N. tangutorun fruit using a UPLC-Q-TOF-MS method.

Abbreviations: DES, deep eutectic solvent; ChCl-Prop, choline chloride/1,2-propanediol.

KEYWORDS:

• Anthocyanins
• Nitraria tangutorun Bobr. fruit
• green extraction
• HPLC-DAD
• UPLC-Q-TOF-MS

Acknowledgements

This work was supported by the Fundamental Research Funds for the Central Universities (GK201603110, GK201706009), the Shaanxi Province Industry-University-Research Cooperative Innovation Project (2017XT-007) and the National Key R&D Program of China (2018YFC1706504). We thank Prof. Zhe-zhi Wang for his generosity in sharing his lab facilities.

Conflict of Interest

All authors declare that they have no conflict of interest to disclose.

Supplementary Material

Supplemental data for this article can be accessed here.

Additional information

Funding

This work was supported by the Fundamental Research Funds for the Central Universities [GK201603110, GK201706009];Shaanxi Province Industry-University-Research Cooperative Innovation Project [2017XT-007];National Key R&D Program of China [2018YFC1706504].