Optimization of ultrasound-assisted aqueous extraction of polyphenols from Psidium guajava leaves using response surface methodology

ABSTRACT

The ultrasound-assisted aqueous extraction of polyphenols from Psidium guajava leaves was investigated using response surface methodology. The total polyphenols yield was significantly affected by the process parameters (ultrasonic power, extraction time, and temperature) and the experimental polyphenols yield at optimal conditions (390.68 W, 38.38 min, 63.23°C) was well agreed with the predicted value (59.82 mg GAE/g). Meanwhile, the aqueous extracts had strong scavenging activity to DPPH. The HPLC analysis showed that gallic acid was the main phenolic component in guava leaves. It was also found that this ultrasound procedure could be successfully applied in industrial production.

KEYWORDS:

• Psidium guajava leaves
• polyphenols
• ultrasound-assisted extraction
• response surface methodology

Additional information

Funding

This work was supported by the National Natural Science Foundation of China under Grant No. 31470230, 51320105006, 51604308, 31100173; the Youth Talent Foundation of Hunan Province of China under Grant No.2017RS3003; Natural Science Foundation of Hunan Province of China under Grant No.2018JJ2486; Fundamental Research Funds for the Central Universities of Central South University under Grant No.2018zzts763.