Optimization of extraction conditions for active ingredients of Suye Huanglian Decoction and its anti-influenza effect in vivo

ABSTRACT

Uniform design method (UD) was performed to define the levels of impact factors. The contents of anti-influenza active ingredients, extraction yield, and the influenza virus inhibition ratio were measured to evaluate the Suye Huanglian Decoction (SHD) aqueous extraction processes. The best optimal extraction process was optimized by genetic algorithm (GA). Further SHD extract was obtained under the optimal extraction process (add 28-fold water, extract for 108 min at 97°C for triple re-extraction). And the pneumonia mouse model established was used to investigate the anti-influenza effect of SHD. The results in vivo showed the optimized SHD exerted an excellent anti-influenza agent.

KEYWORDS:

• Suye Huanglian Decoction
• extraction conditions
• anti-influenza virus
• rosmarinci acid
• berberine hydrochloride
• uniform design-genetic algorithm
• pneumonia

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was funded by Chinese National Natural Science Foundation (No. 81473335/H2803) and Natural Science Foundation of Zhejiang Province (No. LY18H280007).