Improvement in bioactive protein storage stability and colon-targeted release: a simple double-layer chitosan-based particle

Abstract

Aim: To improve the long-term storage stability of a bioactive protein and the subsequent colon-targeted release, a double-layer chitosan (CS)-based particle was developed.

Methods: To form the double-layer CS-based particle, the second layer was crosslinked onto the single-layer bovine serum albumin (BSA)-loaded CS-based particle. The structure and properties of particles were further investigated.

Results: With the second layer, the double-layer particles became more compact, which was important for the inhibition of bioactive protein leakage during storage through strong electrostatic interactions and swelling of the hydrogels. After 30 d of storage, there was only 43.74–49.32% BSA leakage from the C15-TPP/C15-HMP double-layer particles. Moreover, the BSA release in subsequent colon-targeted delivery after storage was 44.02–48.59%.

Conclusions: With double-layer shielding and a more compact arrangement, it was possible to reduce bioactive protein leakage over long periods storage and achieve subsequent colon-targeted delivery.

Keywords:

• Double-layer particle
• crosslinked chitosan
• bioactive protein
• colon-targeted release
• storage stability

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Pearl River S&T Nova Program of Guangzhou under grant No. 201806010184, the National Natural Science Funds of China under grant No. 31601422 and SCAU Student’s Platform for Innovation and Entrepreneurship Training Program under grant No. 201810564116.