The anti-cancer effect of resveratrol nano-encapsulated supplements against breast cancer via the regulation of oxidative stress

Abstract

Aim

Several shreds of evidence have supported the growth-inhibitory effect of resveratrol in breast cancer. Owing to the low efficiency, we aimed to fabricate an ACN nanoparticle containing resveratrol to target the proliferation of breast cancer cells.

Methods

Resveratrol encapsulation was characterized using spectrophotometry, FTIR, and SEM. The cytotoxicity and antioxidant activities of compounds were evaluated by MTT, NO, FRAP, and qRT-PCR assays on MCF7 and SKBr3 cells.

Results

Our result found that the encapsulation efficiency was 87%, the particle size was 200 ± 15 nm, and the zeta potential was 31 ± 0.4 mV. The prepared RES + ACN had controlled in vitro release. RES + ACN nanoparticle showed a significantly increased cytotoxicity in both cell lines. The reduced level of NO and enhance antioxidative content in both cells, especially the MCF7, were in line with increased expression of Nrf2 and SOD as well as more apoptotic effect.

Conclusion

Decreased growth and increased expression of Nrf2 in MCF7 compared to SKBr3 cells proved that the upregulation of Nrf2 by nanoresveratrol is likely contributed to its relationship with ER/PR signaling factors, albeit its precise mechanism is needed to be more elucidated.

Keywords:

• Resveratrol
• nanoencapsulation
• breast cancer
• oxidative stress
• ACN

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

No funding was received for this manuscript.