Corneal Hydration Control during Ex Vivo Experimentation Using Poloxamers

ABSTRACT

Purpose: The purpose of this study was to develop an effective treatment method using poloxamers to restore and maintain physiological hydration in postmortem porcine and human corneas during ex vivo experimentation, and to compare corneal inflation response with or without treatment.

Materials and Methods: Corneal buttons obtained from whole globes (n = 30 porcine, n = 8 human) were treated with various concentrations of poloxamer 188 (P188, a synthetic macromolecule surfactant) for 24 hrs to identify the concentration that would return the cornea to near-physiological hydration (i.e. H = 3.2). Whole globes (n = 12 porcine, n = 16 human) were also used to monitor central corneal thickness (CCT) during deswelling treatment. Inflation testing from 5 to 30 mmHg was performed in the porcine globes and a subset of human globes to characterize the mechanical response of the cornea after treatment.

Results: Physiological hydration was obtained after 24 hrs immersion in 3.25% P188 for porcine corneas and 4.25% P188 treatment for human corneas. CCT was stabilized and returned to physiological levels after 24 hrs of treatment in 3.25% P188 in porcine (891 ± 66 µm) and 4.25% P188 in human (574 ± 34 µm) whole globes. Corneal axial strains at 30 mmHg were significantly larger at physiological hydration than in swollen cornea in both porcine (−6.42%±1.50% vs. −3.64%±1.05%, p = .004) and human (−2.85%±0.09% in vs. −1.53%±0.27%, p = .031) eyes.

Conclusions: Our results suggest that P188 treatment was effective in restoring and maintaining near physiological corneal hydration during ex vivo testing, and hydration appeared to significantly impact corneal inflation response in both porcine and human eyes.

KEYWORDS:

• Corneal hydration
• poloxamers
• corneal deswelling
• inflation testing
• corneal strains

Additional information

Funding

This work was supported by the National Institutes of Health under grant number [EY025358].