A highly sensitive fluorescent probe for hydrazine detection: synthesis, characterisation and application in living cells

ABSTRACT

A new fluorescent probe HSL-AI derived from imidazo [1,5- a]pyridine was synthesised for the detection of hydrazine (N₂H₄) based on excited-state intramolecular proton transfer (ESIPT). HSL-AI was designed using acetyl group as the recognition site and exhibited high selectivity towards N₂H₄ with a low limit of detection (2.56 ppb). Moreover, HSL-AI was successfully applied to detect N₂H₄ in living cells.

KEYWORDS:

• Probe
• Hydrazine
• Imidazo [15-a]pyridine
• Cellular imaging

Disclosure statement

No potential conflict of interest was reported by the authors.

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Additional information

Funding

This study work did not receive any specific grant from funding agencies in the public, commercial or non-profit sectors.