Trace elements and microbiological parameters in farmed Nile tilapia with emphasis on muscle, water, sediment and feed

Abstract

Fish may bioaccumulate contaminants from the aquatic environment and extend them to the food chain provoking risks to human health. This study evaluated the microbiological parameters of the pond´s water and trace elements concentrations in samples of water, sediment, feed and muscle of farmed Nile tilapia used for human consumption in southern Brazil. A total of 240 fish were collected from 12 tilapia farms. Sediment, tank water and dry ration used in the animals' diet were collected for analysis. Analysis were performed by Energy Dispersion X-ray Fluorescence (EDXRF), Inductively Coupled Plasma Mass Spectrometer (ICP-MS), Induced Coupled Plasma Optical Emission Spectrometer (ICP-OES), and Atomic Absorption Spectrometer (AAS-VGA). In addition, the microbiological analysis of the water was carried out. The concentrations of Se, I, Fe, Cu and Zn in fish muscle were higher than the recommended by the Brazilian legislation, considering the advised daily intake for adults. The arsenic element had concentrations above the limit stipulated by the present Brazilian legislation, observed in all samples of muscle, sediment and tank water highlighting a possible environmental and fish contamination by the toxic element. Moreover, the arsenic concentration in the water presented a positive correlation (ρ = 0.33) with arsenic in the muscle, suggesting that tilapia is a good environmental bioindicator, once they properly reflect the levels of arsenic in the water. It is suggested to perform an arsenic speciation for quantification of the inorganic form and accurate assessment of the degree of toxicity in the muscle samples and risks it can bring to human health. Regarding the other potentially toxic elements (Hg, Pb and Cd), and microbiological analysis of water it was verified that the consumption of the fish in question does not raise risks, since the values are within a quality benchmark established by law. The concentration of total and fecal coliforms in pond´s water in the facilities was in agreement with the microbiological indexes required by the legislation of CONAMA class II. Western region presented the lowest concentrations of fecal coliforms when compared to the other regions. There was no significant difference in the microbiological counts of total heterotrophic bacteria, Vibrio spp. and Pseudomonas spp. among the regions.

Keywords:

• Fish farming
• contamination
• sediment
• water
• arsenic
• microbiology

Additional information

Funding

The authors are grateful to the National Council for Scientific and Technological Development (CNPq) for their financial support (CNPq 446072/2014-1) and for granting the Research Productivity grant to M.L. Martins (CNPq 305869/2014-0) and J.L.P. Mouriño (CNPq 308292/2014-6), the Improvement Coordination Higher Level Personnel (CAPES-EMBRAPA n. 15/2014) for the award of the Master’s Scholarship to L.D. Steckert. To Luiz Rodrigo Motta Vicenti (Epagri), Ofélia Maria Campigotto (Gaspar fish farmers’ association), Susane Pahl-Klipp and Roberto Hoppe (Rural Development Foundation July 25), Marcelo Tonial and Alexander Hilata (Nicolluzi) for the assistance in collections and fish donation. Conselho Nacional de Desenvolvimento Científico e Tecnológico; Conselho Nacional de Desenvolvimento Científico e Tecnológico; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.