24-Epicastasterone and KH₂PO₄ protect grain production of wheat crops from terminal heat impacts by modulating leaf physiology

ABSTRACT

Heat stress during the grain filling is one of most critical factors impacting grain yield in winter wheat. In a two-year field experiment, we studied the effects of 24-Epicastasterone and KH₂PO₄ on heat-stressed wheat applied during different times. A completely randomized block design was used with three reagents, that is, 24-Epicastasterone, KH₂PO₄ and H₂O and three spraying times, that is, pre-heat, under-heat and post-heat. Two cultivars, that is, Huaimai 33 and Annong 0711 were subjected to a 7 d post-flowering heat stress using plastic tents. Our study suggests that greater grain weight in heat-stressed plants was achieved through sustaining leaf greenness and higher assimilates supplies to grains. When heat-stressed plants sprayed either with 24-Epicastasterone or KH₂PO₄ could sustain a relatively higher leaf photosynthetic capacity by up-regulating antioxidant enzymes with reduced damage on lipid membranes and leaf chlorophyll. We also found that KH₂PO₄ was relatively more effective in improving leaf physiology and grain yield of heat-stressed plants than 24-Epicastasterone. Further analysis showed that pre-heat or post-heat was more pronounced in protecting plants from heat injury than spraying the plants under heat stress. Thus, spraying KH₂PO₄ either prior to or post stress incidence is recommended to protect wheat crops from terminal heat stress.

KEYWORDS:

• Triticum aestivum L.
• high temperature stress
• spraying reagent
• grain yield

Disclosure statement

No potential conflict of interest was reported by the author(s).

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/03650340.2022.2130265

Additional information

Funding

This study was funded by National Key Research and Development Program of China (2017YFD0300204-3)