Hsa-let-7b Suppresses Cell Proliferation by Targeting UHRF1 in Melanoma

Abstract

UHRF1 promotes melanoma progression by inducing cell proliferation, and is correlated with poor prognosis of melanoma patients. However, the regulation mechanism has not been fully elaborated. Here, we detected hsa-let-7b expression and its role in melanoma. Through Targetscan and miRanda predication, 30 overlapped miRNAs were found; further survival analysis revealed that hsa-let-7b was the only miRNA that affected the overall survival. Overexpressed hsa-let-7b could significantly inhibit the proliferation ability of A375 and A2058 cells, and this phenomenon was reversed after co-transfection with pLenti-UHRF1. In conclusion, hsa-let-7b regulates melanoma cells proliferation in vitro by targeting UHRF1.

Keywords:

• Hsa-let-7b
• UHRF1
• melanoma
• proliferation
• prognosis

Acknowledgments

The authors thank the Pathology Department of Zhongshan Hospital for providing tumor samples.

Ethics approval and consent to participate

Ethical approval for the study was obtained from the Ethics Committee of the Zhongshan Hospital Biomedical Research, and written informed consent was obtained from each patient.

Authors’ contributions

GJY and QFZ conceived and designed the study. LNH and WCY performed the experiments. LNH wrote the manuscript. WCY reviewed and edited the manuscript. All authors read and approved the manuscript and agree to be accountable for all aspects of the research in ensuring that questions about the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.

Availability of data and materials

The datasets used in the present study are available from the corresponding author upon reasonable request.

Additional information

Funding

This study was funded by the National Natural Science Foundation of China [81972559].