Room-temperature-storable chemiluminescence freeze-drying mixes for detection of SARS-CoV-2 neutralizing antibody

Abstract

As the vaccine was successfully developed, the spread of the epidemic (COVID-19) was effectively controlled. But there are still thousands of people affected COVID-19 after being vaccinated. Neutralizing activity has become a critical method for quantifying neutralizing antibody against SARS-CoV-2. However, limited to the strict conditions of cold chain transportation, the neutralizing activity test has not been widely promoted. In this study, a room-temperature-storable chemiluminescence freeze-drying mixes for SARS-CoV-2 neutralizing antibody detection was developed to decrease the cost of lyophilization step for promoting its application in third world countries. Several freeze concentrated solutions were used to protect the antigen bioactivity. The mixes can be stored at room temperature over 12 months and still exhibited great accuracy and precision. Thus, the proposed room-temperature-storable chemiluminescence freeze-drying mixes offers a cheap and stable storage method for SARS-CoV-2 neutralizing antibody detection and shows a great potential for promoting the neutralizing activity test.

Keywords:

• Freeze-drying
• chemiluminescence
• SARS-CoV-2
• neutralizing antibody

Additional information

Funding

This work was supported by the Guangxi Key Research and Development Program (2020AB39270), Guangxi Natural Science Foundation (81873913), National Major Scientific Research Equipment Development Projects (61627807), SARS-CoV-2 prevention and control small highland (Guilin2020), Young Teacher’s basic Ability Promotion Project of Guangxi (2021KY0224), and Innovation Project of Guilin University of Electronic Technology Graduate Education (2021YCXS172).