Abstract
The complex formation between the synthetic water-soluble Zn-meso-tetra(4-N-hydroxyethylpyridyl) porphyrin (ZnTOEPyP4) and cancer DNA in comparison to healthy DNA was investigated using the UV/VIS spectrophotometry method in phosphate-buffered saline at different pHs. The increasing of DNA/porphyrin ratio leads to hypochromicity and red shift in the Soret band, which indicate the complexation of the ZnTOEPyP4 with DNA. The results show that the binding constant () and the exclusion parameter (
) of ZnTOEPyP4 with DNA strongly depend upon the pH. The
of ZnTOEPyP4 with cancer DNA is higher than with normal DNA.
Communicated by Ramaswamy H. Sarma
Disclosure statement
No potential conflict of interest was reported by the author(s).