Mice held at an environmental photic cycle oscillating at their tau-like period length do not show the high-fat diet-induced obesity that develops under the 24-hour photic cycle

ABSTRACT

Circadian disruptions precede high-fat diet (HFD)-induced obesity (DIO). Deviation of the endogenous circadian rhythm period length (tau) from 24 hours correlates with mice inter-strain DIO under the 24-hour light-dark cycle (T-cycle). Additionally, entrainment to a tau-resembling T-cycle attenuates DIO, to some extent, in muted mice. These phenomena suggest that entrainment to a 24-hour T-cycle promotes DIO beyond that expected from the HFD-induced metabolic disruptions. However, the hypothesis that entrainment to a tau-resembling T-cycle attenuates DIO has not been tested in wild-type mice. Therefore, we examined, in newborn female FVB/N mice, whether DIO found under their ‘regular’ 24-hour T-cycle is attenuated under a T-cycle oscillating at their tau-resembling period of 23.7 h, which is diverted from 24 hours by only 0.3 h. Compared to mice fed a low-fat diet, those fed an HFD under the 24-hour T-cycle showed a disrupted pattern of circadian locomotor activity prior to DIO onset. Both these phenomena were absent under the tau-like T-cycle. DIO developed under the 24-hour T-cycle despite similar caloric intake, and was associated with the lower locomotor activity of HFD-fed mice compared to the other mouse groups. These results demonstrated that DIO is secondary to HFD-induced circadian disruptions that are not harmonized by the strongest Zeitgeber (light-dark cycle) when oscillating at a period that diverts by as little as ca. 0.3-h from tau. More importantly, imposing a light-dark cycle oscillating at a tau-like period length, which enhances entrainment and presumably reinforces endogenous circadian rhythms, prevented HFD-induced circadian disruptions and enabled tighter control of energy homeostasis, as manifested by the absence of DIO, even under ad-lib HFD feeding. These results support the identification of tau-related biomarkers, which may be considered as risk-factors for DIO. Moreover, these findings may promote the development of clock-related pharmaceutical interventions that will reduce the gap between tau and 24 hours, and increase the robustness of the endogenous and entrained circadian rhythms. This will enable reducing DIO, even without caloric restriction or time-restricted feeding.

KEYWORDS:

• Circadian rhythms
• locomotor activity
• obesity
• circadian period length
• circadian disruption
• Zeitgeber
• high-fat diet
• tau

Acknowledgements

The research leading to these results received funding from the German-Israel Foundation for Scientific Research and Development; and the European Union’s Seventh Framework Programme FP7-REGPOT-2012-2013-1, Agreement n. 316157. The authors thank Ateret Shabtay-Yanai, Maayan Rauch, Mariel Alejandra Pinsky, Hila Yehuda, Naama Tal, and Bat Chan Ackerman for their assistance in conducting the experiments, and Haim Cohen from Bar-Ilan University, Ramat-Gan, Israel, for providing access to his NMR for measuring body mass composition in mice.

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This work was supported by the German-Israeli Foundation for Scientific Research and Development [I-2337-203.13/2014]; Seventh Framework Programme, European Union research and development funding programme [FP7-REGPOT-2012-2013-1, Agreement n. 316157].