IP-10 and MCP-1 gene polymorphisms in Chinese patients with chronic immune thrombocytopenia

Abstract

Aberrant Th1/Th2 polarization is considered to play a crucial role in the abnormal immune state of primary immune thrombocytopenia (ITP). IFN-γ-inducible protein of 10 kilodaltons (IP-10) and Monocyte chemoattractant protein-1 (MCP-1) gene are involved in enhancing the Th1 and Th2 immune response, respectively. In this study we investigated the distributions of IP-10 (-201 G/A) and MCP-1 (-2518 A/G) polymorphisms in 323 patients with chronic ITP and 255 healthy controls by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). The IP-10 and MCP-1 levels of blood serum from 79 adult ITP patients and 43 healthy controls were detected with ELISA. The frequency of AG + AA genotype in IP-10 (-201 G/A) was significantly higher in ITP patients than in controls, especially in female and adult patients. ITP patients showed higher IP-10 levels than normal controls. Moreover, both IP-10 (-201 G/A) heterozygote (GA) and homozygote minor allele (AA) patients had significantly increased IP-10 levels compared to homozygote genotype (GG) patients at diagnosis. No significant differences were revealed in genotypes and allele distributions of MCP-1 (-2518 A/G) between ITP patients and normal controls, as well as the MCP-1 levels. In conclusion, the -201 G/A polymorphism of IP-10 gene may be associated with the susceptibility of ITP in Chinese population.

Keywords:

• Immune thrombocytopenia
• polymorphism
• IP-10
• MCP-1
• pathogenesis

Acknowledgements

The authors would like to thank Dr. Hao Zhang for assistance with the statistical analysis.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by grants of National Natural Science Foundation of China (81700115, 81670118) and the Fundamental Research Funds for the Central Universities (2042016kf0103).