Abdominal Ischemia-Reperfusion Induced Cardiac Dysfunction Can Be Prevented by MitoTEMPO

Abstract

Background

Cardiac dysfunction is secondary to acute mesenteric ischemia (AMI) and abdominal aortic aneurysms (AAA). The underlying cause of distant organ damage in the heart is the formation of oxidative stress caused by ischemia-reperfusion. In this study, we investigated the possible protective effects of a novel mitochondria-targeted antioxidant MitoTEMPO on contractile dysfunction and structural defects of the rat papillary muscle caused by abdominal ischemia-reperfusion (AIR).

Methods and Results

In the experiments, adult Wistar-Albino rats were used and animals were divided randomly into 3 groups; sham-operated group (SHAM), an IR group that had aortic cross-clamping for 1 h followed by 2 h reperfusion, and a third group that received protective 0.7 mg/kg/day MitoTEMPO injection for 28-day before IR. As a result, it was observed that MitoTEMPO injection had a protective effect on the mechanical activities and structural properties of the papillary muscle impaired by AIR. Our study also showed that AIR disrupted the contractile function of the papillary muscle for each stimulation frequency and post-potentiation responses tested. This is common for each measured and calculated mechanical parameter and MitoTEMPO injection showed its protective effects.

Conclusion

Consequently, calcium homeostasis seems to be impaired by AIR, and MitoTEMPO may exert its protective effect through energy metabolism by directly targeting the mitochondria.

Keywords:

• MitoTEMPO
• papillary muscle
• ischemia-reperfusion
• muscle contraction

Author’s contributions

Substantial contributions to the conception or design of the work; or the acquisition, analysis, or interpretation of data for the work (Ahmet Akkoca, Murat Cenk Celen, Seckin Tuncer, and Nizamettin Dalkilic). Drafting the work or revising it critically for important intellectual content (Ahmet Akkoca, Murat Cenk Celen, and Seckin Tuncer). Final approval of the version to be published (Ahmet Akkoca, Murat Cenk Celen, Seckin Tuncer, and Nizamettin Dalkilic). Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved (Ahmet Akkoca, Murat Cenk Celen, and Seckin Tuncer).

Disclosure statement

No potential conflict of interest was reported by the authors.