Investigations on the influence of the structural flexibility of nanoliposomes on their properties

Abstract

In the present study, nanoliposomes with tuneable structure elasticity were prepared by reverse-phase evaporation. Both Fluorescence Polarization and Fluorescence Resonance Energy Transfer was employed to characterize the structural elasticity of resultant nanoliposomes. Temozolomide, a kind of hydrophilic drug as the first-line treatment choice for glioblastoma, was encapsulated into nanoliposomes. The results showed that the flexibility of nanoliposomes gradually increased with sodium cholate, while decreasing with cholesterol, Labrafac CC and Labrafac PG adding. Furthermore, the structural flexibility of nanoliposomes was positively correlated with the encapsulation efficiency and release rate and cellular uptake. Our research reveals the structural flexibility of nanoliposomes could affect in vitro characteristics and thereafter in vivo behaviors of nanoliposomes.

Keywords:

• Structural flexibility
• nanoliposomes
• encapsulation efficiency
• in vitro release
• cytotoxicity
• cellular uptake

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors gratefully acknowledge financial supports from National Natural Science Foundation of China [No. 81803471], High-level health talents in Jiangsu Province “Six One Project” Top-notch Talents Scientific Research Project [LGY2019026] and Natural Science Foundation of the Chinese Traditional Medical University of Nanjing [No. XZR2020042].