Abstract
The regeneration of bone defects is a major challenge for clinical orthopaedics. Herein, we designed and prepared a new type of bioactive material, using stingray skin collagen and oyster shell powder (OSP) as raw materials. A stingray skin collagen/oyster osteoinductive composite scaffold (Col-OSP) was prepared for the first time by genipin cross-linking, pore-forming and freeze-drying methods. These scaffolds were characterized by ATR-FTIR, SEM, compression, swelling, cell proliferation, cell adhesion, alkaline phosphatase activity, alizarin red staining and RT-PCR etc. The Col-OSP scaffold had an interconnected three-dimensional porous structure, and the mechanical properties of the Col-OSP composite scaffold were enhanced compared with Col, combining with the appropriate swelling rate and degradation rate, the scaffold was more in line with the requirements of bone tissue engineering scaffolds. The Col-OSP scaffold was non-toxic, promoted the proliferation, adhesion, and differentiation of MC3T3-E1 cells, and stimulated the osteogenesis-related genes expressions of osteocalcin (OCN), collagen type I (COL-I) and RUNX2 of MC3T3-E1 cells.
Author contributions
Yue Wu: Investigation, Visualization, Data curation, Writing-Reviewing and Editing. Yingkun Fu: Visualization, Investigation. Hongfu Pan: Visualization, Investigation. Cong Chang: Visualization, Investigation. Ningjian Ao: Visualization, Investigation. Hui Xu: Visualization, Investigation. Zhengnan Zhang: Software, Validation. Ping Hu: Software, Validation. Riwang Li: Software, Validation. Shuxia Duan: Supervision, Project administration. Yan Yan Li: Conceptualization, Methodology, Writing-Reviewing and Editing.
Disclosure statement
No potential conflict of interest was reported by the authors.
Ethical approval
The animal experiments were completed according to the protocols approved by the Laboratory Animal Center, Jinan University, Guangzhou, China. All animal care and treatment were approved by the Laboratory Animal Care Agency.