322
Views
7
CrossRef citations to date
0
Altmetric
Original Article

Phenotype of Innate Immune Cells in Uveitis Associated with Axial Spondyloarthritis- and Juvenile Idiopathic Arthritis-associated Uveitis

, PhDORCID Icon, , MD, , PhDORCID Icon, , PhDORCID Icon, , PhDORCID Icon, , PhD, , PhD, , PhDORCID Icon, , MD, , MD & , MDORCID Icon show all
Pages 1080-1089 | Received 02 Aug 2019, Accepted 09 Jan 2020, Published online: 11 Mar 2020
 

ABSTRACT

Purpose: To analyze circulating immune cells in patients with anterior uveitis (AU) associated to axial spondyloarthritis (SpA), or juvenile idiopathic arthritis (JIA).

Methods: Venous blood samples were collected from healthy controls (n = 16), and either SpA (n = 19) or JIA (n = 23) patients with associated anterior uveitis (AU) during active flare, or after ≥3 months of inactivity. Frequencies of CD56+, MHC-I+, and S100A9+ monocytes, CCR7+ dendritic cells, CD56+dim natural killer (NK) cells and CD3+CD56bright T-cells were analyzed via flow cytometry. Serum S100A8/A9 levels were determined via ELISA.

Results: SpA patients showed a reduced frequency of CD56+dim NK cells during uveitis activity, a constitutively activated monocyte phenotype, and elevated S100A8/A9 serum levels. In contrast, JIAU patients showed elevated frequencies of CD56+ monocytes and CCR7+ DC.

Conclusion: Phenotype of peripheral immune cells differ between patients, probably contributing to different courses of acute onset AU in SpA and insidious onset AU in JIAU patients.

Abbreviations: AU: anterior uveitis, AR: arthritis, JIA: juvenile idiopathic arthritis, SpA: axial spondyloarthritis

Acknowledgments

We thank Prof. Dr. med. C. Heinz and the medical team of the department of ophthalmology at St. Franziskus Hospital, B. Stratmann, L. Jacubeck, E. Attenberger, B. Zurek-Imhoff. We thank Dr. med. R. Walscheid (MVZ for laboratory medicine, Koblenz, Germany) for conducting the HLA-B27 PCR analysis.

Authors´ contributions

MK, KW, DB, MB, TL, and AH designed the study and wrote the manuscript. MK, BL, DB, MB, KL, TV performed the experiments and analyzed the data. KW, GG, TR, and AH collected the peripheral blood samples and provided clinical data.

Competing interests

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary material

Supplemental data for this article can be accessed on the publisher’s website.

Additional information

Funding

The current study was in part funded by Deutsche Forschungsgemeinschaft (DFG) He 1877/19-1; part of the FOR 2240 program (Lymph) Angiogenesis and cellular immunity in inflammatory diseases of the eye/project 8, uveitis.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 65.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 815.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.