The Rs12569232 SNP Association with Vogt-Koyanagi-Harada Disease and Behcet’s Disease is Probably Mediated by Regulation of Linc00467 Expression

ABSTRACT

Purpose: To investigate whether the rs12569232 SNP association with Vogt-Koyanagi-Harada disease and Behcet’s disease is mediated by regulation of Linc00467 expression.

Methods: The expression of linc00467 was detected by real-time PCR. Adenovirus carrying the linc00467 was transduced into CD4⁺T cells and the effect on cell viability was measured by the CCK-8 test. Human proteome microarray and starBase 2.0 were used to identify the binding proteins of linc00467 and RNA Immunoprecipitation (RIP) was used to confirm the identity of bound proteins.

Results: The rs12569232 was associated with the expression of linc00467. The expression of linc00467 was up-regulated in PBMCs and CD4⁺T cells from VKH disease and BD patients. Over-expression of linc00467 increased cell viability of CD4⁺T cells. HUR was the common binding protein identified by the two methods and confirmed by RIP.

Conclusions: The rs12569232 association with VKH disease and BD may be mediated via regulating the expression of linc00467.

KEYWORDS:

• Vogt-Koyanagi-Harada disease
• Behcet’s disease
• lincRNA
• Cd4⁺T cells
• single nucleotide polymorphism

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Supplementary material

Supplemental data for this article can be accessed on the publisher’s website.

Additional information

Funding

This study was supported by National Natural Science Foundation Key Program (81930023), Natural Science Foundation Major International (Regional) Joint Research Project (81720108009), Chongqing Outstanding Scientists (2019), Chongqing Key Laboratory of Ophthalmology (CSTC, 2008CA5003), Chongqing Science & Technology Platform and Base Construction Program (cstc2014pt-sy10002) and the Natural Science Foundation Project of Chongqing (cstc2017shmsA130073).