Mixed B- and T-lymphocyte Vitreous Infiltrate in Multiple Sclerosis Associated Uveitis

ABSTRACT

A 23-year-old man, under treatment for relapsing remitting multiple sclerosis, presented with sudden drop in vision in the left eye for the past 1 week. We noted optic atrophy with sclerosed vessels in multiple quadrants in both eyes, moderate vitreous haze, and active retinal vasculitis in left eye. The patient received therapeutic pars plana vitrectomy in the left eye, and the vitreous sample was analyzed for immunophenotypes by flow cytometry (T-cells) and immunohistochemistry (B-cells). 65.1% of total vitreous cells were CD3⁺ T-cells. These included 42.4% CD4⁺, and 20.6% CD8⁺ T-cells. Immunohistochemistry detected CD20⁺ B-cells (not quantifiable). Our analysis demonstrated a mixed B- and T-lymphocyte vitreous infiltrate in multiple sclerosis-associated uveitis.

KEYWORDS:

• B-cell
• immunophenotype
• multiple sclerosis
• T-cell
• uveitis
• vitreous

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

DBT-Wellcome Trust India Alliance Intermediate Fellowship in Clinical and Public Health Research (SB, #IA/CPHI/18/1/503975); Hyderabad Eye Research Foundation