Identification of mutation in TMB1M6 gene in response to heat stress in goats

ABSTRACT

BAX inhibitor-1 (BI-1) gene, known as TMB1M6, is evolutionary conserved and regulates cell survival in response to various stress in animals and plants. The present study was carried out to analyse the TMB1M6 gene in relation to stress susceptible and tolerant phenotypes in different goat breeds by high resolution melting (HRM). Phenotyping of individuals were carried out basing on heart rate (HR) and respiration rate (RR) during peak heat stress period and recognized as heat stress-tolerant (HST) and heat stress-susceptible (HSS). Genotyping of TMB1M6 gene was carried out by HRM. HRM  indicated four different groups in the analysed samples. Two novel mutations were observed in heat stress-tolerant and stress-susceptible individuals in analysed samples. The nucleotide polymorphisms were identified at base position 55(T>C) in heat stress-tolerant (HST) individual in Barbari goat. Similarly the mutation at base position 96 was observed in heat stress-susceptible (HSS) individual in Jakhrana goat. The nucleotide sequence analysis indicated that the percent similarity of TMBIM 6 gene fragment of goat with cattle, human and mouse was 92.2, 85.2 and 80.9%, respectively. Two SNPs were identified  in heat stress susceptible and heat stress tolerant phenotype in goat.

KEYWORDS:

• Bax inhibitor-1
• high-resolution melting
• SNP

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The work was funded through project no. C4/C-30033 (comp-IV), NAIP, Indian Council of Agricultural Research, New Delhi, India.