Comparison of the effect of isotretionin and alitretionin on endometriotic implants and serum vascular endothelial growth factor level: an experimental study

Abstract

Objective: To compare the effects of alitretionin and isotretionin on endometrial peritoneal implants and serum vascular endothelial growth factor (VEGF) levels.

Study design: Forty-eight female Sprague Dawley rats were used. Initially surgical rat endometriosis model was done. The endometrial implant volume was measured and rats were randomly divided into four groups. Group 1: Control group (rats did not get any drug but having endometriotic implants), group 2: rats receiving po isotretionin 10 mg/kg per day for 10 d, group 3: rats receiving po isotretionin 20 mg/kg per day for 10 d and group 4: rats receiving po alitretionin 80 mg/kg per day for 10 d. After 1-week medication, rats were sacrificed and size, histopathology of endometriotic implant and levels of VEGF were evaluated.

Results: Volumes of peritoneal endometrial implants were significantly decreased in Group 2 and Group 3 compared with initial values. However, there were no significant changes in histopathological scores and serum VEGF levels in all groups.

Conclusions: This study finding may suggest the possible medical treatment modality of isotretionin on endometriosis. However, alitretionin (potent retinoid) does not have potent regressive effect on endometriotic implants as in isotretionin.

摘要

目的：比较异维A酸和阿米替林对子宫内膜异位症腹膜植入物及血清血管内皮生长因子（VEGF）水平的影响

研究设计：采用48只雌性SD大鼠。首先通过手术建造大鼠子宫内膜异位症模型。测量子宫内膜异位植入物的体积, 将大鼠随机分为四组。组1：对照组（没有给予任何药物但有子宫内膜异位植入物）；组2：每天灌胃给予10mg / kg异维A酸, 持续10天；组3：每天灌胃给予20mg / kg异维A酸, 持续10天；组4：每天灌胃给予80mg / kg阿米替林, 持续10天。给药结束一周后, 处死大鼠, 并检测子宫内膜异位植入物的大小、组织病理学和血清中VEGF水平。

结果：组2和组3中子宫内膜异位腹膜植入物的体积相比于初始值明显增加。然而, 组织病理学评分和血清VEGF水平在各组之间无明显差别。

结论：本研究的发现提示异维A酸在子宫内膜异位症中可能的内科治疗方式。然而, 阿米替林（有效的维甲酸）对于子宫内膜异位植入物没有像异维A酸一样强效的退化作用。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• Alitretionin
• endometriosis
• isotretionin
• retinoid
• VEGF

Disclosure statement

No potential conflict of interests was reported by the author(s).

Additional information

Funding

This study was financially supported by the Unit of Karadeniz Technical University medical report project (project number 11701)