Relationship between adipocytokines and angiotensin converting enzyme gene insertion/deletion polymorphism in lean women with and without polycystic ovary syndrome

Abstract

This study was designed to investigate the relationship between the levels of select adipocytokines (adiponectin, visfatin and apelin) and angiotensin in converting enzyme (ACE) gene insertion/deletion (ID) polymorphism in lean women with and without polycystic ovary syndrome (PCOS). The PCOS group (N = 94) was identified according to the Rotterdam criteria. The Control group (N = 68) included age- and body mass index (BMI)-matched healthy volunteers. Serum levels of adipocytokines were measured using enzyme immunoassays (EIA) and ACE genes were evaluated by polymerase chain reaction (PCR). The PCOS group, when compared to the Control group had lower adiponectin (p < .001) but higher visfatin (p < .001) and apelin (p = .003). There was no significant correlation of the levels of these adipocytokines with BMI, fasting glucose, fasting insulin or Homeostasis Model Assessment-Insulin Resistance (HOMA-IR). The PCOS and the Control groups also differed with regard to the ACE ID genotype distribution (p < .001). The ID, DD, and II genotype frequencies were, respectively, 34, 57 and 9% in the PCOS group and 49, 22 and 29% in the Control group. When stratified according to individual ID genotypes, the levels of adipocytokines in the PCOS and the Control groups remained significantly different. There was no statistically significant relationship between the levels of adipocytokines and ACE ID genotypes.

摘要

本研究旨在研究患有和未患有多囊卵巢综合征（PCOS）的瘦妇女的选择脂肪细胞因子（脂联素, 内脏脂肪酸和apelin）水平与血管紧张素转化酶（ACE）基因插入/缺失（ID）多态性之间的关系。PCOS组（n=94）根据鹿特丹标准确定。对照组(n=68)包括年龄和体重指数（BMI）相匹配的健康志愿者。采用酶免疫分析法（EIA）检测血清脂肪细胞因子水平, 采用聚合酶链反应（PCR）检测ACE基因水平。与对照组相比, 多囊卵巢综合征组的脂联素水平较低（p<0.001）, 但内脏脂肪素水平（p<0.001）和apelin（p=.003）较高。这些脂肪细胞因子的水平与BMI、空腹血糖、空腹胰岛素或稳态模型评估-胰岛素抵抗（HOMA-IR）无显著相关性。PCOS与对照组在ACE基因型分布上也存在差异（p < .001）。PCOS组的ID、DD、II基因型频率分别为34、57、9%；对照组的ID、DD、II基因型频率分别为49、22、29%。按个体ID基因型分层时, PCOS组和对照组的脂肪细胞因子水平仍存在显著差异。脂肪细胞因子水平与ACE基因型无统计学意义。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• Polycystic ovary syndrome
• adipocytokines
• angiotensin converting enzyme
• polymorphism

Disclosure statement

The authors report no conflict of interest.

Additional information

Funding

This work was supported by Grant for Young Scientists of the Poznan University of Medical Sciences.