HNF1A gene p.I27L is associated with co-existing preeclampsia in gestational diabetes mellitus

Abstract

The association of the FTO gene and HNF1α gene on gestational diabetes mellitus (GDM) and preeclampsia remains unclear. This is the first study to examine whether HNF1α gene and FTO gene were associated with having GDM and preeclampsia in Turkish women. Healthy pregnant women (n = 101) and women with GDM (n = 169) were included. GDM was divided into two groups as GDM-only (n = 90) and GDM-preeclampsia (n = 79). Genotyping of HNF1α gene p.I27L, p.A98V, and p.S487N, and FTO gene rs9939609 SNPs were performed using RT-PCR. The frequency of p.S487N, p.A98V, and FTO genotype were similar between the groups (p > .05). p.I27L GG-wild, GT, and TT genotype were 56.5%, 36.6%, and 6.9% in controls; 40.0%, 51.1%, and 8.9% in GDM-only; and 26.6%, 51.9%, and 21.5% in GDM-preeclampsia (p = .034). TT and GT genotype was more frequent in GDM-preeclampsia than in controls (p < .05). GT genotype was increased in GDM-only compared with controls (p < .05). TT genotype was more frequent in GDM-preeclampsia than in GDM-only (p < .05). p.I27L TT genotype was independently associated with increased blood pressure (BP) and urinary protein. p.I27L TT genotype was associated with increased preeclampsia risk in patients with GDM by increasing BP and urinary protein.

摘要

FTO基因和HNF1α基因与妊娠期糖尿病（GDM）和先兆子痫的关系尚不清楚。这是首次研究HNF1α基因和FTO基因是否与土耳其妇女患有妊娠期糖尿病和先兆子痫有关。包括健康孕妇（n=101例）和妊娠期糖尿病妇女（n=169例）。妊娠期糖尿病分为单纯妊娠期糖尿病组（n=90例）和妊娠期糖尿病先兆子痫组（n=79例）。采用RT-PCR方法对HNF1α基因p.I27L、p.A98V、p.S487N和FTO基因rs9939609SNPs进行基因分型。两组间p.S487N、p.A98V和FTO基因型频率相似（p>0.05）。PI27L GG-Wild、GT和TT基因型在对照组分别为56.5%、36.6%和6.9%；在单纯GDM组分别为40.0%、51.1%和8.9%；在GDM先兆子痫组分别为26.6%、51.9%和21.5%（p=.034）。GDM-子痫前期组TT和GT基因型频率明显高于对照组（p<0.05）。与对照组相比, 仅GDM组GT基因型增加（p<0.05）。TT型在妊娠期糖尿病先兆子痫组中的频率高于单纯妊娠期糖尿病组（p<0.05）。P.I27L TT基因型与血压(BP)和尿蛋白升高独立相关。在妊娠期糖尿病患者中, P.I27L TT基因型通过升高血压和尿蛋白而增加先兆子痫风险。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• HNF1α
• FTO
• p.I27L
• preeclampsia
• gestational diabetes

Disclosure statement

The authors declare that they have no competing interests.

Availability of data and materials

All data are freely available for scientific purposes.